Templated DNA ligation with thiol chemistry

Dadong Li, Xiaojian Wang, Fubo Shi, Ruojie Sha, Nadrian Seeman, James Canary

Research output: Contribution to journalArticle

Abstract

We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner. This journal is

Original languageEnglish (US)
Pages (from-to)8823-8827
Number of pages5
JournalOrganic and Biomolecular Chemistry
Volume12
Issue number44
DOIs
StatePublished - Nov 28 2014

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Sulfhydryl Compounds
thiols
Ligation
deoxyribonucleic acid
disulfides
chemistry
Disulfides
DNA
Chemical reactions
chemical reactions
templates
catalysts
Catalysts
products

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Biochemistry
  • Medicine(all)

Cite this

Templated DNA ligation with thiol chemistry. / Li, Dadong; Wang, Xiaojian; Shi, Fubo; Sha, Ruojie; Seeman, Nadrian; Canary, James.

In: Organic and Biomolecular Chemistry, Vol. 12, No. 44, 28.11.2014, p. 8823-8827.

Research output: Contribution to journalArticle

Li, Dadong ; Wang, Xiaojian ; Shi, Fubo ; Sha, Ruojie ; Seeman, Nadrian ; Canary, James. / Templated DNA ligation with thiol chemistry. In: Organic and Biomolecular Chemistry. 2014 ; Vol. 12, No. 44. pp. 8823-8827.
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