Telencephalic neural progenitors appear to be restricted to regional and glial fates before the onset of neurogenesis

Maria McCarthy, Daniel H. Turnbull, Christopher A. Walsh, Gordon Fishell

Research output: Contribution to journalArticle

Abstract

The contribution of early cell lineage to regional fate in the mammalian forebrain remains poorly understood. Previous lineage-tracing studies using retroviral methods were only begun at mid-neurogenesis and have suffered from region-specific retroviral silencing. We have been able to study cell lineage in the telencephalon from the onset of neurogenesis by using ultrasound backscatter microscopy to label the forebrain neuroepithelium and a modified retroviral lineage library to overcome regional silencing. Our studies suggest that by embryonic day 9.5, forebrain clones are primarily restricted to territories within anatomically demarcated regional boundaries, such as the cortex, striatum and hypothalamus. In addition, we observed a subset of clones that appeared to be composed entirely of glia. These observations suggest that both regional and cell-type restrictions exist within progenitor populations before the first forebrain cells become postmitotic.

Original languageEnglish (US)
Pages (from-to)6772-6781
Number of pages10
JournalJournal of Neuroscience
Volume21
Issue number17
StatePublished - Sep 1 2001

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Telencephalon
Neurogenesis
Prosencephalon
Neuroglia
Cell Lineage
Clone Cells
Hypothalamus
Libraries
Microscopy
Population

Keywords

  • Cell identity
  • Lineal analysis
  • Neurogenesis
  • Regional restriction
  • Retrovirus
  • Telencephalon

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Telencephalic neural progenitors appear to be restricted to regional and glial fates before the onset of neurogenesis. / McCarthy, Maria; Turnbull, Daniel H.; Walsh, Christopher A.; Fishell, Gordon.

In: Journal of Neuroscience, Vol. 21, No. 17, 01.09.2001, p. 6772-6781.

Research output: Contribution to journalArticle

McCarthy, Maria ; Turnbull, Daniel H. ; Walsh, Christopher A. ; Fishell, Gordon. / Telencephalic neural progenitors appear to be restricted to regional and glial fates before the onset of neurogenesis. In: Journal of Neuroscience. 2001 ; Vol. 21, No. 17. pp. 6772-6781.
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