Technique to collect fungiform (Taste) papillae from human tongue

Andrew Spielman, M. Yanina Pepino, Roy Feldman, Joseph G. Brand

Research output: Contribution to journalArticle

Abstract

The sense of taste is critical for human life. It informs the body about the quality of food that will be potentially ingested and stimulates metabolic processes that prepare the alimentary canal for digestion. Steady progress is being made towards understanding the early biochemical and molecular events underlying taste transduction (for a review, Breslin and Spector, 2008). However, progress to date has largely resulted from animal models. Yet, since marked differences in receptor specificity and receptor density vary among species, human taste transduction will only be understood by using human taste tissue. Here we describe a biopsy technique to collect human fungiform papillae, visible as rounded pink anterior structures, about 0.5 mm in diameter that contain taste buds. These biopsied papillae are used for several purposes including the isolation of viable taste bud cells, in situ hybridization, immunohistochemistry and, through techniques of molecular biology, the identification of taste-specific novel proteins.

Original languageEnglish (US)
Article numbere2201
JournalJournal of visualized experiments : JoVE
Issue number42
DOIs
StatePublished - Aug 2010

Fingerprint

Molecular biology
Biopsy
Canals
Tongue
Animals
Tissue
Proteins
Taste Buds
Food Quality
In Situ Hybridization
Molecular Biology
Digestion
Animal Models
Immunohistochemistry

Keywords

  • Biopsy
  • Fungiform papillae
  • Human
  • Issue 42
  • JoVE Medicine
  • Taste cells
  • Tongue

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Technique to collect fungiform (Taste) papillae from human tongue. / Spielman, Andrew; Yanina Pepino, M.; Feldman, Roy; Brand, Joseph G.

In: Journal of visualized experiments : JoVE, No. 42, e2201, 08.2010.

Research output: Contribution to journalArticle

@article{4ee3cccc443e48f8b1c8c234284bb5c2,
title = "Technique to collect fungiform (Taste) papillae from human tongue",
abstract = "The sense of taste is critical for human life. It informs the body about the quality of food that will be potentially ingested and stimulates metabolic processes that prepare the alimentary canal for digestion. Steady progress is being made towards understanding the early biochemical and molecular events underlying taste transduction (for a review, Breslin and Spector, 2008). However, progress to date has largely resulted from animal models. Yet, since marked differences in receptor specificity and receptor density vary among species, human taste transduction will only be understood by using human taste tissue. Here we describe a biopsy technique to collect human fungiform papillae, visible as rounded pink anterior structures, about 0.5 mm in diameter that contain taste buds. These biopsied papillae are used for several purposes including the isolation of viable taste bud cells, in situ hybridization, immunohistochemistry and, through techniques of molecular biology, the identification of taste-specific novel proteins.",
keywords = "Biopsy, Fungiform papillae, Human, Issue 42, JoVE Medicine, Taste cells, Tongue",
author = "Andrew Spielman and {Yanina Pepino}, M. and Roy Feldman and Brand, {Joseph G.}",
year = "2010",
month = "8",
doi = "10.3791/2201",
language = "English (US)",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "42",

}

TY - JOUR

T1 - Technique to collect fungiform (Taste) papillae from human tongue

AU - Spielman, Andrew

AU - Yanina Pepino, M.

AU - Feldman, Roy

AU - Brand, Joseph G.

PY - 2010/8

Y1 - 2010/8

N2 - The sense of taste is critical for human life. It informs the body about the quality of food that will be potentially ingested and stimulates metabolic processes that prepare the alimentary canal for digestion. Steady progress is being made towards understanding the early biochemical and molecular events underlying taste transduction (for a review, Breslin and Spector, 2008). However, progress to date has largely resulted from animal models. Yet, since marked differences in receptor specificity and receptor density vary among species, human taste transduction will only be understood by using human taste tissue. Here we describe a biopsy technique to collect human fungiform papillae, visible as rounded pink anterior structures, about 0.5 mm in diameter that contain taste buds. These biopsied papillae are used for several purposes including the isolation of viable taste bud cells, in situ hybridization, immunohistochemistry and, through techniques of molecular biology, the identification of taste-specific novel proteins.

AB - The sense of taste is critical for human life. It informs the body about the quality of food that will be potentially ingested and stimulates metabolic processes that prepare the alimentary canal for digestion. Steady progress is being made towards understanding the early biochemical and molecular events underlying taste transduction (for a review, Breslin and Spector, 2008). However, progress to date has largely resulted from animal models. Yet, since marked differences in receptor specificity and receptor density vary among species, human taste transduction will only be understood by using human taste tissue. Here we describe a biopsy technique to collect human fungiform papillae, visible as rounded pink anterior structures, about 0.5 mm in diameter that contain taste buds. These biopsied papillae are used for several purposes including the isolation of viable taste bud cells, in situ hybridization, immunohistochemistry and, through techniques of molecular biology, the identification of taste-specific novel proteins.

KW - Biopsy

KW - Fungiform papillae

KW - Human

KW - Issue 42

KW - JoVE Medicine

KW - Taste cells

KW - Tongue

UR - http://www.scopus.com/inward/record.url?scp=80355140578&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80355140578&partnerID=8YFLogxK

U2 - 10.3791/2201

DO - 10.3791/2201

M3 - Article

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 42

M1 - e2201

ER -