Targeted Control of Kinetics of β-Amyloid Self-association by Surface Tension-modifying Peptides

Jin Ryoun Kim, Todd J. Gibson, Regina M. Murphy

Research output: Contribution to journalArticle

Abstract

Brain tissue from Alzheimer's patients contains extracellular senile plaques composed primarily of deposits of fibrillar aggregates of β-amyloid peptide. β-Amyloid aggregation is postulated to be a major factor in the onset of this neurodegenerative disease. Recently proposed is the hypothesis that oligomeric intermediates, rather than fully formed insoluble fibrils, are cytotoxic. Previously, we reported the discovery of peptides that accelerate β-amyloid aggregation yet inhibit toxicity in vitro, in support of this hypothesis. These peptides contain two domains: a recognition element designed to bind to β-amyloid and a disrupting element that alters β-amyloid aggregation kinetics. Here we show that the aggregation rate-enhancing activity of the disrupting element correlates strongly with its ability to increase surface tension of aqueous solutions. Using the Hofmeister series as a guide, we designed a novel peptide with terminal side-chain trimethylammonium groups in the disrupting domain. The derivatized peptide greatly increased solvent surface tension and accelerated β-amyloid aggregation kinetics by severalfold. Equivalent increases in surface tension in the absence of a recognition domain had no effect on β-amyloid aggregation. These results suggest a novel strategy for targeting localized changes in interfacial energy to specific proteins, as a way to selectively alter protein folding, stability, and aggregation.

Original languageEnglish (US)
Pages (from-to)40730-40735
Number of pages6
JournalJournal of Biological Chemistry
Volume278
Issue number42
DOIs
StatePublished - Oct 17 2003

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Surface Tension
Amyloid
Surface tension
Agglomeration
Association reactions
Peptides
Kinetics
Neurodegenerative diseases
Protein folding
Protein Stability
Protein Folding
Amyloid Plaques
Interfacial energy
Neurodegenerative Diseases
Toxicity
Brain
Deposits
Tissue

ASJC Scopus subject areas

  • Biochemistry

Cite this

Targeted Control of Kinetics of β-Amyloid Self-association by Surface Tension-modifying Peptides. / Kim, Jin Ryoun; Gibson, Todd J.; Murphy, Regina M.

In: Journal of Biological Chemistry, Vol. 278, No. 42, 17.10.2003, p. 40730-40735.

Research output: Contribution to journalArticle

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