Targeted amplification of delivery to cell surface receptors by dendrimer self-assembly

Steven Isaacman, Michael Buckley, Xiaojian Wang, Edwin Y. Wang, Leonard Liebes, James Canary

Research output: Contribution to journalArticle

Abstract

Nanometer-scale architectures assembled on cell surface receptors from smaller macromolecular constituents generated a large amplification of fluorescence. A targeted dendrimer was synthesized from a cystamine-core G4 PAMAM dendrimer, and contained an anti-BrE3 monoclonal antibody as the targeting group, several fluorophores and an average of 12 aldehyde moieties as complementary bio-orthogonal reactive sites for the covalent assembly. A cargo dendrimer, derived from a PAMAM G4 dendrimer, contained several fluorophores as the cargo for delivery and five hydrazine moieties as complimentary bio-orthogonal reactive sites. The system is designed to be flexible and allow for facile incorporation of a variety of targeting ligands.

Original languageEnglish (US)
Pages (from-to)1290-1293
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume24
Issue number5
DOIs
StatePublished - Mar 1 2014

Fingerprint

Dendrimers
hydrazine
Cell Surface Receptors
Self assembly
Amplification
Catalytic Domain
Fluorophores
Cystamine
Aldehydes
Fluorescence
Monoclonal Antibodies
Ligands
PAMAM Starburst
PAMAM-G4

Keywords

  • Cell surface self-assembly
  • Dendrimer
  • Targeted amplification

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry
  • Biochemistry

Cite this

Targeted amplification of delivery to cell surface receptors by dendrimer self-assembly. / Isaacman, Steven; Buckley, Michael; Wang, Xiaojian; Wang, Edwin Y.; Liebes, Leonard; Canary, James.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 24, No. 5, 01.03.2014, p. 1290-1293.

Research output: Contribution to journalArticle

Isaacman, Steven ; Buckley, Michael ; Wang, Xiaojian ; Wang, Edwin Y. ; Liebes, Leonard ; Canary, James. / Targeted amplification of delivery to cell surface receptors by dendrimer self-assembly. In: Bioorganic and Medicinal Chemistry Letters. 2014 ; Vol. 24, No. 5. pp. 1290-1293.
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