Tapping mode atomic force microscopy of the hyaluronan derivative, hylan A

Mary Cowman, M. Li, A. Dyal, E. A. Balazs

Research output: Contribution to journalArticle

Abstract

Hylan A, a water-soluble hyaluronan derivative with enhanced molecular weight attributed to protein-mediated crosslinks, has been examined by tapping mode atomic force microscopy (TMAFM). Hylan A was deposited on mica from dilute aqueous solution and imaged in air through a thin layer of adsorbed water. Isolated chains, drawn into extended forms to varying degrees by the movement of a droplet interface across the surface ('molecular combing'), were observed. The majority of the extended chains could be characterized as long linear single-stranded molecules, as is observed for hyaluronan. In a smaller number of observations on extended hylan A, it was possible to observe apparent connections between two chains, presumably mediated by covalent linkage via protein. Hylan A was also commonly observed in less extended forms. Isolated chains of this type often exhibited intramolecular association, the simplest form of which was antiparallel double-stranded (probably double-helical) junction zones. More extensive intramolecular association, via intermittent double- or multi-stranded connections, led to a fenestrated appearance. Intermolecular association was also observed, leading to the formation of network-like matrices. The variety and types of structures observed for hylan A by TMAFM were in excellent agreement with predictions based on physicochemical studies of both hylan A and hyaluronan.

Original languageEnglish (US)
Pages (from-to)229-235
Number of pages7
JournalCarbohydrate Polymers
Volume41
Issue number3
DOIs
StatePublished - Mar 2000

Fingerprint

hyaluronic acid
atomic force microscopy
Atomic Force Microscopy
Hyaluronic Acid
Atomic force microscopy
chemical derivatives
Derivatives
Proteins
Mica
Water
mica
Molecular weight
droplets
Molecules
aqueous solutions
linkage (genetics)
proteins
Air
water
molecular weight

ASJC Scopus subject areas

  • Food Science
  • Biochemistry

Cite this

Tapping mode atomic force microscopy of the hyaluronan derivative, hylan A. / Cowman, Mary; Li, M.; Dyal, A.; Balazs, E. A.

In: Carbohydrate Polymers, Vol. 41, No. 3, 03.2000, p. 229-235.

Research output: Contribution to journalArticle

Cowman, Mary ; Li, M. ; Dyal, A. ; Balazs, E. A. / Tapping mode atomic force microscopy of the hyaluronan derivative, hylan A. In: Carbohydrate Polymers. 2000 ; Vol. 41, No. 3. pp. 229-235.
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