Synthetic Control of Tertiary Helical Structures in Short Peptides

Michael G. Wuo, Seong Ho Hong, Arunima Singh, Paramjit S. Arora

Research output: Contribution to journalArticle

Abstract

Helical secondary and tertiary motifs are commonly observed as binding epitopes in natural and engineered protein scaffolds. While several strategies have been described to constrain α-helices or reproduce their binding attributes in synthetic mimics, general strategies to mimic tertiary helical motifs remain in their infancy. We recently described a synthetic strategy to develop helical dimers (J. Am. Chem. Soc. 2015, 137, 11618-11621). We found that replacement of an interhelical salt bridge with a covalent bond can stabilize antiparallel motifs in short sequences. Here we show that the approach can be generalized to obtain antiparallel and parallel dimers as well as trimer motifs. Helical stabilization requires judiciously designed cross-linkers as well as optimal interhelical hydrophobic packing. We anticipate that these mimics would afford new classes of modulators of biological function.

Original languageEnglish (US)
Pages (from-to)16284-16290
Number of pages7
JournalJournal of the American Chemical Society
Volume140
Issue number47
DOIs
StatePublished - Nov 28 2018

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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