Synthesis from DNA of a molecule with the connectivity of a cube

Research output: Contribution to journalArticle

Abstract

A principal goal of biotechnology is the assembly of novel biomaterials for analytical, industrial and therapeutic purposes. The advent of stable immobile nucleic acid branched junctions makes DNA a candidate for building frameworks to which proteins or other functional molecules can be attached and thereby juxtaposed. The addition of single-stranded 'sticky' ends to branched DNA molecules converts them into macromolecular valence clusters that can be ligated together. The edges of these frameworks are double-helical DNA, and the vertices correspond to the branch points of junctions. Here, we report the construction from DNA of a covalently closed cube-like molecular complex containing twelve equal-length double-helical edges arranged about eight vertices. Each of the six 'faces' of the object is a single-stranded cyclic molecule, doubly catenated to four neighbouring strands, and each vertex is connected by an edge to three others. Each edge contains a unique restriction site for analytical purposes. This is the first construction of a closed polyhedral object from DNA.

Original languageEnglish (US)
Pages (from-to)631-633
Number of pages3
JournalNature
Volume350
Issue number6319
DOIs
StatePublished - 1991

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DNA
Biocompatible Materials
Biotechnology
Nucleic Acids
Proteins
Therapeutics

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Synthesis from DNA of a molecule with the connectivity of a cube. / Chen, J.; Seeman, Nadrian.

In: Nature, Vol. 350, No. 6319, 1991, p. 631-633.

Research output: Contribution to journalArticle

Chen, J. ; Seeman, Nadrian. / Synthesis from DNA of a molecule with the connectivity of a cube. In: Nature. 1991 ; Vol. 350, No. 6319. pp. 631-633.
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