Synthesis and characterization of nanoscale-hydroxyapatite-copper for antimicrobial activity towards bone tissue engineering applications

K. Sahithi, M. Swetha, M. Prabaharan, A. Moorthi, N. Saranya, K. Ramasamy, N. Srinivasan, Nicola Partridge, N. Selvamurugan

Research output: Contribution to journalArticle

Abstract

The bacterial infection is one of the major problems associated with implant and reconstructive surgery of bone. Hence, the aim of this study was to develop biomaterials having antibacterial activity for bone tissue engineering. The hydroxyapatite nanoparticles (nHAp) improve the mechanical properties and incorporate nanotopographic features that mimic the nanostructure of natural bone. We report here for the first time the synthesis and characterization of nHAp and nHAp soaked with copper (nHAp-Cu) using SEM, AFM, FTIR and XRD. The antibacterial activity of nHAp and nHAp-Cu was determined using Gram-positive and Gram-negative bacterial strains. To have accelerated antibacterial activity, polyethylene glycol 400 (PEG 400), a synthetic biodegradable polymer was also added along with nHAp-Cu. The nHAp-Cu/PEG 400 had increased antibacterial activity towards Gram-positive than Gram-negative bacterial strains. The cytotoxicity of nHAp-Cu/PEG 400 was determined using MTT assay with rat primary osteoprogenitor cells and these biomaterials were found to be non-toxic. Hence, based on these results we suggest that the biomaterials containing nHAp-Cu/PEG 400 can be used as antibacterial materials in bone implant and bone regenerative medicine.

Original languageEnglish (US)
Pages (from-to)333-339
Number of pages7
JournalJournal of Biomedical Nanotechnology
Volume6
Issue number4
DOIs
StatePublished - Aug 2010

Fingerprint

Durapatite
Tissue Engineering
Hydroxyapatite
Tissue engineering
Copper
Bone
Biocompatible Materials
Polyethylene glycols
Bone and Bones
Biomaterials
Reconstructive Surgical Procedures
Regenerative Medicine
Nanostructures
Fourier Transform Infrared Spectroscopy
Bacterial Infections
Biodegradable polymers
Nanoparticles
Cytotoxicity
Polymers
Surgery

Keywords

  • Antibacterial activity
  • Nano-hydroxyapatite particles
  • Nano-hydroxyapatite soaked with copper
  • Polyethylene glycol

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Cite this

Synthesis and characterization of nanoscale-hydroxyapatite-copper for antimicrobial activity towards bone tissue engineering applications. / Sahithi, K.; Swetha, M.; Prabaharan, M.; Moorthi, A.; Saranya, N.; Ramasamy, K.; Srinivasan, N.; Partridge, Nicola; Selvamurugan, N.

In: Journal of Biomedical Nanotechnology, Vol. 6, No. 4, 08.2010, p. 333-339.

Research output: Contribution to journalArticle

Sahithi, K. ; Swetha, M. ; Prabaharan, M. ; Moorthi, A. ; Saranya, N. ; Ramasamy, K. ; Srinivasan, N. ; Partridge, Nicola ; Selvamurugan, N. / Synthesis and characterization of nanoscale-hydroxyapatite-copper for antimicrobial activity towards bone tissue engineering applications. In: Journal of Biomedical Nanotechnology. 2010 ; Vol. 6, No. 4. pp. 333-339.
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