Fonction des genes Pax: Synergie de liaison a l'ADN entre le domaine paired et l'homeodomaine

Translated title of the contribution: Synergism in DNA binding of paired and homeo domains in Pax protein

Research output: Contribution to journalArticle

Abstract

The Pax genes encode a set of transcriptional regulators involved in several developmental processes. They are characterized by the presence of the paired domain (PD), a DNA binding domain composed of two Helix-Turn-Helix (HTH) motifs, the PAI and RED domains. Some Pax proteins contain a third HTH DNA binding motif, the homeodomain (HD). Since all PDs recognize highly related DNA sites, and since all HDs recognize a common TAAT sequence, it has been difficult to understand how these proteins achieve their functional specificity. Here, we describe how different Pax proteins use multiple combinations of their DNA binding motifs to target different promoters. In vitro, the Drosophila paired protein can bind either through its PAI domain, through its HD via cooperative dimerization, or through both domains. In vivo, using a transgenic rescue assay, we show that prd function requires the synergistic action of both the PAI domain and the HD on abutted PD and HD sites. Surprisingly, the RED domain, although conserved, can be deleted without loss of viability. This is in contrast to other Pax proteins that require both PAI and RED domains. Furthermore, specific isoforms of Pax6 as well as a new Pax protein, Lune, may rely on the RED domain alone. Finally, we propose that Pax6 may also act through its HD alone on a series of highly conserved palindromic TAAT sites found in all rhodopsin promoters. This may represent the ancient function of this master regulator of eye development before it acquired a PD.

Original languageFrench
Pages (from-to)147-155
Number of pages9
JournalMedecine/Sciences
Volume13
Issue number2
StatePublished - Feb 1997

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Polyamideimides
DNA
Nucleotide Motifs
Proteins
Helix-Turn-Helix Motifs
Rhodopsin
Dimerization
Assays
Protein Isoforms
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fonction des genes Pax : Synergie de liaison a l'ADN entre le domaine paired et l'homeodomaine. / Desplan, Claude.

In: Medecine/Sciences, Vol. 13, No. 2, 02.1997, p. 147-155.

Research output: Contribution to journalArticle

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