Surface vs. solution hybridization: Effects of salt, temperature, and probe type

Wanqiong Qiao, Hao Chun Chiang, Hui Xie, Rastislav Levicky

Research output: Contribution to journalArticle

Abstract

Hybridization thermodynamics on solid supports are compared with those in solution for two types of hybridization probe, DNA and uncharged morpholino oligonucleotides of identical sequences. Trends in hybridization affinity are discussed with respect to ionic strength, temperature, and surface behavior.

Original languageEnglish (US)
Pages (from-to)17245-17248
Number of pages4
JournalChemical Communications
Volume51
Issue number97
DOIs
StatePublished - Oct 1 2015

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Morpholinos
Oligonucleotides
DNA Probes
Ionic strength
DNA
Salts
Thermodynamics
Temperature

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys

Cite this

Surface vs. solution hybridization : Effects of salt, temperature, and probe type. / Qiao, Wanqiong; Chiang, Hao Chun; Xie, Hui; Levicky, Rastislav.

In: Chemical Communications, Vol. 51, No. 97, 01.10.2015, p. 17245-17248.

Research output: Contribution to journalArticle

Qiao, Wanqiong ; Chiang, Hao Chun ; Xie, Hui ; Levicky, Rastislav. / Surface vs. solution hybridization : Effects of salt, temperature, and probe type. In: Chemical Communications. 2015 ; Vol. 51, No. 97. pp. 17245-17248.
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