Suppressor of hairless activates Brachyury expression the Ciona embryo

Joseph C. Corbo, Shigeki Fujiwara, Michael Levine, Anna Di Gregorio

Research output: Contribution to journalArticle

Abstract

The Ciona Brachyury gene (Ci-Bra) is regulated, in part, by a 434-bp enhancer that mediates restricted expression in the notochord. Here we present evidence that a Ciona Suppressor of Hairless {Ci-Su(H)} protein functions as an activator of this enhancer. Point mutations that reduce the binding of a GST/Ci-Su(H) fusion protein in vitro diminish the expression of mutagenized Ci-Bra/lacZ transgenes in electroporated embryos. Overexpression of a Ci-Su(H) fusion protein containing the Drosophila Hairy repression domain interferes with notochord differentiation, producing mutant tadpoles with shortened tails. Expression of a constitutively activated Xotch receptor in the notochord, endoderm, and CNS also alters tail morphogenesis. These results suggest that a Notch-Su(H) pathway might participate in notochord differentiation in Ciona.

Original languageEnglish (US)
Pages (from-to)358-368
Number of pages11
JournalDevelopmental Biology
Volume203
Issue number2
DOIs
StatePublished - Nov 15 1998

Fingerprint

Notochord
Embryonic Structures
Activator Appliances
Endoderm
Lac Operon
Transgenes
Morphogenesis
Point Mutation
Larva
Proteins
Brachyury protein
Genes

Keywords

  • Brachyury
  • Ciona intestinalis
  • Notochord
  • Suppressor of Hairless

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Suppressor of hairless activates Brachyury expression the Ciona embryo. / Corbo, Joseph C.; Fujiwara, Shigeki; Levine, Michael; Di Gregorio, Anna.

In: Developmental Biology, Vol. 203, No. 2, 15.11.1998, p. 358-368.

Research output: Contribution to journalArticle

Corbo, Joseph C. ; Fujiwara, Shigeki ; Levine, Michael ; Di Gregorio, Anna. / Suppressor of hairless activates Brachyury expression the Ciona embryo. In: Developmental Biology. 1998 ; Vol. 203, No. 2. pp. 358-368.
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