Abstract
The T 1 of a hyperpolarized site in solution is a key parameter that determines the time-window in which its NMR signals are observable. For 13 C sites adjacent to protons, 1 H-decoupling has been shown to increase the hyperpolarized signal resolution and SNR. Additionally, polarization transfer to protons has shown utility in increasing the sensitivity of detection. However, 1 H-decoupling could lead to a change in the decay rate of a hyperpolarized 13 C site. Here we tested this possible effect in a case where the protons are directly bound to an sp 3 hyperpolarized 13 C site (using [1,2- 13 C 2 ]choline) and 1 H-decoupling was applied continuously throughout the hyperpolarized decay measurement. We found that 1 H-decoupling did not lead to any significant changes in the 13 C polarization decay time but did result in the expected collapse of J-coupling and produced sharper signals. This result suggested that 1 H-decoupling did not affect the decay rate of hyperpolarized sp 3 13 C sites. The deuterium-substitution approach (using [1,1,2,2-D 4 ,1- 13 C]choline) showed a dramatic prolongation of T 1 . Upper bounds on the T 1 of all investigated sites were calculated.
| Original language | English (US) |
|---|---|
| Journal | Israel Journal of Chemistry |
| DOIs | |
| State | Published - Jan 1 2019 |
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ASJC Scopus subject areas
- Chemistry(all)
Cite this
1 H-decoupling and Isotopic Labeling for the Measurement of the Longitudinal Relaxation Time of Hyperpolarized 13 C-Methylenes in Choline Analogs . / Uppala, Sivaranjan; Gamliel, Ayelet; Harris, Talia; Sosna, Jacob; Gomori, J. Moshe; Jerschow, Alexej; Katz-Brull, Rachel.
In: Israel Journal of Chemistry, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 1 H-decoupling and Isotopic Labeling for the Measurement of the Longitudinal Relaxation Time of Hyperpolarized 13 C-Methylenes in Choline Analogs
AU - Uppala, Sivaranjan
AU - Gamliel, Ayelet
AU - Harris, Talia
AU - Sosna, Jacob
AU - Gomori, J. Moshe
AU - Jerschow, Alexej
AU - Katz-Brull, Rachel
PY - 2019/1/1
Y1 - 2019/1/1
N2 - The T 1 of a hyperpolarized site in solution is a key parameter that determines the time-window in which its NMR signals are observable. For 13 C sites adjacent to protons, 1 H-decoupling has been shown to increase the hyperpolarized signal resolution and SNR. Additionally, polarization transfer to protons has shown utility in increasing the sensitivity of detection. However, 1 H-decoupling could lead to a change in the decay rate of a hyperpolarized 13 C site. Here we tested this possible effect in a case where the protons are directly bound to an sp 3 hyperpolarized 13 C site (using [1,2- 13 C 2 ]choline) and 1 H-decoupling was applied continuously throughout the hyperpolarized decay measurement. We found that 1 H-decoupling did not lead to any significant changes in the 13 C polarization decay time but did result in the expected collapse of J-coupling and produced sharper signals. This result suggested that 1 H-decoupling did not affect the decay rate of hyperpolarized sp 3 13 C sites. The deuterium-substitution approach (using [1,1,2,2-D 4 ,1- 13 C]choline) showed a dramatic prolongation of T 1 . Upper bounds on the T 1 of all investigated sites were calculated.
AB - The T 1 of a hyperpolarized site in solution is a key parameter that determines the time-window in which its NMR signals are observable. For 13 C sites adjacent to protons, 1 H-decoupling has been shown to increase the hyperpolarized signal resolution and SNR. Additionally, polarization transfer to protons has shown utility in increasing the sensitivity of detection. However, 1 H-decoupling could lead to a change in the decay rate of a hyperpolarized 13 C site. Here we tested this possible effect in a case where the protons are directly bound to an sp 3 hyperpolarized 13 C site (using [1,2- 13 C 2 ]choline) and 1 H-decoupling was applied continuously throughout the hyperpolarized decay measurement. We found that 1 H-decoupling did not lead to any significant changes in the 13 C polarization decay time but did result in the expected collapse of J-coupling and produced sharper signals. This result suggested that 1 H-decoupling did not affect the decay rate of hyperpolarized sp 3 13 C sites. The deuterium-substitution approach (using [1,1,2,2-D 4 ,1- 13 C]choline) showed a dramatic prolongation of T 1 . Upper bounds on the T 1 of all investigated sites were calculated.
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U2 - 10.1002/ijch.201900016
DO - 10.1002/ijch.201900016
M3 - Article
AN - SCOPUS:85065104846
JO - Israel Journal of Chemistry
JF - Israel Journal of Chemistry
SN - 0021-2148
ER -