Studying the effects of reproductive hormones and bacterial vaginosis on the glycome of lavage samples from the cervicovaginal cavity

Linlin Wang, Sujeethraj Koppolu, Catherine Chappell, Bernard J. Moncla, Sharon L. Hillier, Lara Mahal

Research output: Contribution to journalArticle

Abstract

The cervicovaginal fluid (CVF) coating the vaginal epithelium is an important immunological mediator, providing a barrier to infection. Glycosylation of CVF proteins, such as mucins, IgG and S-IgA, plays a critical role in their immunological functions. Although multiple factors, such as hormones and microflora, may influence glycosylation of the CVF, few studies have examined their impact on this important immunological fluid. Herein we analyzed the glycosylation of cervicovaginal lavage (CVL) samples collected from 165 women under different hormonal conditions including: (1) no contraceptive, post-menopausal, (2) no contraceptive, days 1-14 of the menstrual cycle, (3) no contraceptive, days 15-28 of the menstrual cycle, (4) combined-oral contraceptive pills for at least 6 months, (5) depomedroxyprogesterone acetate (Depo-Provera) injections for at least 6 months, (6) levonorgestrel IUD for at least 1 month. Glycomic profiling was obtained using our lectin microarray system, a rapid method to analyze carbohydrate composition. Although some small effects were observed due to hormone levels, the major influence on the glycome was the presence of an altered bacterial cohort due to bacterial vaginosis (BV). Compared to normal women, samples from women with BV contained lower levels of sialic acid and high-mannose glycans in their CVL. The change in high mannose levels was unexpected and may be related to the increased risk of HIV-infection observed in women with BV, as high mannose receptors are a viral entry pathway. Changes in the glycome were also observed with hormonal contraceptive use, in a contraceptive-dependent manner. Overall, microflora had a greater impact on the glycome than hormonal levels, and both of these effects should be more closely examined in future studies given the importance of glycans in the innate immune system.

Original languageEnglish (US)
Article numberA1676
JournalPLoS One
Volume10
Issue number5
DOIs
StatePublished - May 20 2015

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Bacterial Vaginosis
contraceptives
Therapeutic Irrigation
Contraceptive Agents
Glycosylation
hormones
Hormones
glycosylation
mannose
menstrual cycle
Fluids
Mannose
Menstrual Cycle
Polysaccharides
sampling
polysaccharides
Glycomics
Contraceptives, Oral, Combined
oral contraceptives
microorganisms

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Studying the effects of reproductive hormones and bacterial vaginosis on the glycome of lavage samples from the cervicovaginal cavity. / Wang, Linlin; Koppolu, Sujeethraj; Chappell, Catherine; Moncla, Bernard J.; Hillier, Sharon L.; Mahal, Lara.

In: PLoS One, Vol. 10, No. 5, A1676, 20.05.2015.

Research output: Contribution to journalArticle

Wang, Linlin ; Koppolu, Sujeethraj ; Chappell, Catherine ; Moncla, Bernard J. ; Hillier, Sharon L. ; Mahal, Lara. / Studying the effects of reproductive hormones and bacterial vaginosis on the glycome of lavage samples from the cervicovaginal cavity. In: PLoS One. 2015 ; Vol. 10, No. 5.
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