Studies on the Rate of Selective Uptake of Amino Acids by Trichinella Larvae in Vivo

T. Alkarmi, M. K. Ijaz, F. K. Dar, Sehamuddin Galadari, S. Alharbi, M. Alnaaib, S. Abdo

Research output: Contribution to journalArticle

Abstract

Groups of C57BL/6J mice, orally infected with 300 larvae each of Trichinella spiralis or T. pseudospiralis were injected with [3H]-alanine, tyrosine, tryptophan or glycine. The incorporation of isotope labelled amino acids into larval proteins was measured at 2, 6, and 12 months post-infection. It was shown that there is a significant increase in the in vivo uptake of isotope labelled amino acids with time by the larvae of T. spiralis and T. pseudospiralis. The level of uptake was highest for tyrosine followed by tryptophan, alanine and then glycine, for both species. The in vivo uptake of amino acids by T. pseudospiralis larvae was always higher than T. spiralis or the host at 6 and 12 months post-infection. At 2 months post-infection, T. spiralis uptake of these amino acids was higher, except for tyrosine. This may be related to the special needs of these larvae during the process of encystation. The higher metabolic requirements of T. pseudospiralis may be related to the higher energy needs of these non encapsulated, highly motile and mobile muscle larvae.

Original languageEnglish (US)
Pages (from-to)273-276
Number of pages4
JournalJournal of Helminthology
Volume68
Issue number4
DOIs
StatePublished - Jan 1 1994

Fingerprint

Trichinella
Trichinella spiralis
Larva
uptake mechanisms
tyrosine
Amino Acids
amino acids
larvae
Tyrosine
glycine (amino acid)
tryptophan
alanine
isotopes
Tryptophan
Isotopes
Alanine
Glycine
Trichinella pseudospiralis
infection
muscle larvae

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology

Cite this

Studies on the Rate of Selective Uptake of Amino Acids by Trichinella Larvae in Vivo. / Alkarmi, T.; Ijaz, M. K.; Dar, F. K.; Galadari, Sehamuddin; Alharbi, S.; Alnaaib, M.; Abdo, S.

In: Journal of Helminthology, Vol. 68, No. 4, 01.01.1994, p. 273-276.

Research output: Contribution to journalArticle

Alkarmi, T. ; Ijaz, M. K. ; Dar, F. K. ; Galadari, Sehamuddin ; Alharbi, S. ; Alnaaib, M. ; Abdo, S. / Studies on the Rate of Selective Uptake of Amino Acids by Trichinella Larvae in Vivo. In: Journal of Helminthology. 1994 ; Vol. 68, No. 4. pp. 273-276.
@article{f7d49d4b18f346f085e705690130ac16,
title = "Studies on the Rate of Selective Uptake of Amino Acids by Trichinella Larvae in Vivo",
abstract = "Groups of C57BL/6J mice, orally infected with 300 larvae each of Trichinella spiralis or T. pseudospiralis were injected with [3H]-alanine, tyrosine, tryptophan or glycine. The incorporation of isotope labelled amino acids into larval proteins was measured at 2, 6, and 12 months post-infection. It was shown that there is a significant increase in the in vivo uptake of isotope labelled amino acids with time by the larvae of T. spiralis and T. pseudospiralis. The level of uptake was highest for tyrosine followed by tryptophan, alanine and then glycine, for both species. The in vivo uptake of amino acids by T. pseudospiralis larvae was always higher than T. spiralis or the host at 6 and 12 months post-infection. At 2 months post-infection, T. spiralis uptake of these amino acids was higher, except for tyrosine. This may be related to the special needs of these larvae during the process of encystation. The higher metabolic requirements of T. pseudospiralis may be related to the higher energy needs of these non encapsulated, highly motile and mobile muscle larvae.",
author = "T. Alkarmi and Ijaz, {M. K.} and Dar, {F. K.} and Sehamuddin Galadari and S. Alharbi and M. Alnaaib and S. Abdo",
year = "1994",
month = "1",
day = "1",
doi = "10.1017/S0022149X00001486",
language = "English (US)",
volume = "68",
pages = "273--276",
journal = "Journal of Helminthology",
issn = "0022-149X",
publisher = "Cambridge University Press",
number = "4",

}

TY - JOUR

T1 - Studies on the Rate of Selective Uptake of Amino Acids by Trichinella Larvae in Vivo

AU - Alkarmi, T.

AU - Ijaz, M. K.

AU - Dar, F. K.

AU - Galadari, Sehamuddin

AU - Alharbi, S.

AU - Alnaaib, M.

AU - Abdo, S.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Groups of C57BL/6J mice, orally infected with 300 larvae each of Trichinella spiralis or T. pseudospiralis were injected with [3H]-alanine, tyrosine, tryptophan or glycine. The incorporation of isotope labelled amino acids into larval proteins was measured at 2, 6, and 12 months post-infection. It was shown that there is a significant increase in the in vivo uptake of isotope labelled amino acids with time by the larvae of T. spiralis and T. pseudospiralis. The level of uptake was highest for tyrosine followed by tryptophan, alanine and then glycine, for both species. The in vivo uptake of amino acids by T. pseudospiralis larvae was always higher than T. spiralis or the host at 6 and 12 months post-infection. At 2 months post-infection, T. spiralis uptake of these amino acids was higher, except for tyrosine. This may be related to the special needs of these larvae during the process of encystation. The higher metabolic requirements of T. pseudospiralis may be related to the higher energy needs of these non encapsulated, highly motile and mobile muscle larvae.

AB - Groups of C57BL/6J mice, orally infected with 300 larvae each of Trichinella spiralis or T. pseudospiralis were injected with [3H]-alanine, tyrosine, tryptophan or glycine. The incorporation of isotope labelled amino acids into larval proteins was measured at 2, 6, and 12 months post-infection. It was shown that there is a significant increase in the in vivo uptake of isotope labelled amino acids with time by the larvae of T. spiralis and T. pseudospiralis. The level of uptake was highest for tyrosine followed by tryptophan, alanine and then glycine, for both species. The in vivo uptake of amino acids by T. pseudospiralis larvae was always higher than T. spiralis or the host at 6 and 12 months post-infection. At 2 months post-infection, T. spiralis uptake of these amino acids was higher, except for tyrosine. This may be related to the special needs of these larvae during the process of encystation. The higher metabolic requirements of T. pseudospiralis may be related to the higher energy needs of these non encapsulated, highly motile and mobile muscle larvae.

UR - http://www.scopus.com/inward/record.url?scp=0028578654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028578654&partnerID=8YFLogxK

U2 - 10.1017/S0022149X00001486

DO - 10.1017/S0022149X00001486

M3 - Article

VL - 68

SP - 273

EP - 276

JO - Journal of Helminthology

JF - Journal of Helminthology

SN - 0022-149X

IS - 4

ER -