Structure of apamin in solution

A two-dimensional nuclear magnetic resonance study

David Wemmer, Neville R. Kallenbach

Research output: Contribution to journalArticle

Abstract

A two-dimensional (2-D) Fourier-transform nuclear magnetic resonance (NMR) study of the 18 amino acid neurotoxin apamin isolated from honeybee venom is reported. Combining 2-D J-correlated spectra with 2-D nuclear Overhauser spectra in H2O solution permits essentially complete assignment of the 1H NMR spectrum of apamin at a fixed pH, including a number of spin systems that are reported for the first time. The structural model previously derived by Bystrov et al. [Bystrov, V. F., Okhanov, V. V., Miroshnikov, A. I., & Ovchinnikov, Yu. A. (1980) FEBS Lett. 119, 113-116] from NMR data is shown to be largely correct. The 2-D nuclear Overhauser effect (NOE) spectrum in particular reveals a series of amide-amide NOE's consistent with an α-helical core (residues 9-15) in the molecule. NOE's between amide and Cα protons, followed by amide to amide NOE's, indicate a β-turn involving residues 3-5 and a nonstandard turn including residues 6-8. We find no evidence for the β-type structure postulated at the C terminus, however. Instead, it appears that the α-helix continues with increasing fraying from residues 16-18.

Original languageEnglish (US)
Pages (from-to)1901-1906
Number of pages6
JournalBiochemistry
Volume22
Issue number8
StatePublished - 1983

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Apamin
Amides
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Structural Models
Venoms
Neurotoxins
Fourier Analysis
Protons
Fourier transforms
Amino Acids
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure of apamin in solution : A two-dimensional nuclear magnetic resonance study. / Wemmer, David; Kallenbach, Neville R.

In: Biochemistry, Vol. 22, No. 8, 1983, p. 1901-1906.

Research output: Contribution to journalArticle

Wemmer, D & Kallenbach, NR 1983, 'Structure of apamin in solution: A two-dimensional nuclear magnetic resonance study', Biochemistry, vol. 22, no. 8, pp. 1901-1906.
Wemmer, David ; Kallenbach, Neville R. / Structure of apamin in solution : A two-dimensional nuclear magnetic resonance study. In: Biochemistry. 1983 ; Vol. 22, No. 8. pp. 1901-1906.
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