Structural study of rat thyroid transcription factor 1 homeodomain (TTF-1 HD) by nuclear magnetic resonance

Paolo Viglino, Federico Fogolari, Silvestro Formisano, Nadia Bortolotti, Giuseppe Damante, Roberto Di Lauro, Gennaro Esposito

Research output: Contribution to journalArticle

Abstract

The 500 MHz 1H NMR spectrum of a 68-residue peptide, encompassing the rat thyroid transcription factor 1 homeodomain (TTF-1 HD), was fully assigned using standard 2D NMR methodology. The secondary structure elements and their spatial organization were determined and led to a structure very similar to that previously described for other homeodomains and expected also for TTF-1 HD from homology modeling predictions. The three-dimensional arrangement of the three helix fragments of TTF-1 HD preserves the helix-tum-helix motif commonly occurring in many classes of DNA-binding proteins.

Original languageEnglish (US)
Pages (from-to)397-402
Number of pages6
JournalFEBS Letters
Volume336
Issue number3
DOIs
StatePublished - Dec 28 1993

Fingerprint

Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
DNA-Binding Proteins
Peptides
thyroid nuclear factor 1
Proton Magnetic Resonance Spectroscopy

Keywords

  • Homeodomain
  • Protein NMR
  • Thyroid transcription factor 1 (TTF-1)

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Structural study of rat thyroid transcription factor 1 homeodomain (TTF-1 HD) by nuclear magnetic resonance. / Viglino, Paolo; Fogolari, Federico; Formisano, Silvestro; Bortolotti, Nadia; Damante, Giuseppe; Di Lauro, Roberto; Esposito, Gennaro.

In: FEBS Letters, Vol. 336, No. 3, 28.12.1993, p. 397-402.

Research output: Contribution to journalArticle

Viglino, P, Fogolari, F, Formisano, S, Bortolotti, N, Damante, G, Di Lauro, R & Esposito, G 1993, 'Structural study of rat thyroid transcription factor 1 homeodomain (TTF-1 HD) by nuclear magnetic resonance', FEBS Letters, vol. 336, no. 3, pp. 397-402. https://doi.org/10.1016/0014-5793(93)80845-L
Viglino P, Fogolari F, Formisano S, Bortolotti N, Damante G, Di Lauro R et al. Structural study of rat thyroid transcription factor 1 homeodomain (TTF-1 HD) by nuclear magnetic resonance. FEBS Letters. 1993 Dec 28;336(3):397-402. https://doi.org/10.1016/0014-5793(93)80845-L
Viglino, Paolo ; Fogolari, Federico ; Formisano, Silvestro ; Bortolotti, Nadia ; Damante, Giuseppe ; Di Lauro, Roberto ; Esposito, Gennaro. / Structural study of rat thyroid transcription factor 1 homeodomain (TTF-1 HD) by nuclear magnetic resonance. In: FEBS Letters. 1993 ; Vol. 336, No. 3. pp. 397-402.
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