Structural insights into the recognition of cisplatin and AAF-dG lesion by Rad14 (XPA)

Sandra C. Koch, Jochen Kuper, Karola L. Gasteiger, Nina Simon, Ralf Strasser, David Eisen, Simon Geiger, Sabine Schneider, Caroline Kisker, Thomas Carell, Suse Broyde

Research output: Contribution to journalArticle

Abstract

Nucleotide excision repair (NER) is responsible for the removal of a large variety of structurally diverse DNA lesions. Mutations of the involved proteins cause the xeroderma pigmentosum (XP) cancer predisposition syndrome. Although the general mechanism of the NER process is well studied, the function of the XPA protein, which is of central importance for successful NER, has remained enigmatic. It is known, that XPA binds kinked DNA structures and that it interacts also with DNA duplexes containing certain lesions, but the mechanism of interactions is unknown. Here we present two crystal structures of the DNA binding domain (DBD) of the yeast XPA homolog Rad14 bound to DNA with either a cisplatin lesion (1,2-GG) or an acetylaminofluorene adduct (AAF-dG). In the structures, we see that two Rad14 molecules bind to the duplex, which induces DNA melting of the duplex remote from the lesion. Each monomer interrogates the duplex with a β-hairpin, which creates a 13mer duplex recognition motif additionally characterized by a sharp 70° DNA kink at the position of the lesion. Although the 1,2-GG lesion stabilizes the kink due to the covalent fixation of the crosslinked dG bases at a 90° angle, the AAF-dG fully intercalates into the duplex to stabilize the kinked structure.

Original languageEnglish (US)
Pages (from-to)8272-8277
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number27
DOIs
StatePublished - Jul 7 2015

Keywords

  • AAF
  • Cisplatin
  • NER
  • Rad14
  • XPA

ASJC Scopus subject areas

  • General

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    Koch, S. C., Kuper, J., Gasteiger, K. L., Simon, N., Strasser, R., Eisen, D., Geiger, S., Schneider, S., Kisker, C., Carell, T., & Broyde, S. (2015). Structural insights into the recognition of cisplatin and AAF-dG lesion by Rad14 (XPA). Proceedings of the National Academy of Sciences of the United States of America, 112(27), 8272-8277. https://doi.org/10.1073/pnas.1508509112