Structural characterization of synthetic model peptides of the DNA‐binding cI434 repressor by electrospray ionization and fast atom bombardment mass spectrometry

Piergiorgio Percipalle, Rosaria Saletti, Sandor Pongor, Salvatore Foti, Alessandro Tossi, Salvatore Fisichella

    Research output: Contribution to journalArticle

    Abstract

    The structural characterization of two synthetic model peptides of the cI434 repressor is described. Unequivocal determination of the structure was achieved by means of electrospray ionization mass spectrometry of the intact peptides and by fast atom bombardment mass spectrometric identification of complementary peptide fragments obtained by tryptic and chymotrypic digestion and partial separation by reversed‐phase high‐performance liquid chromatography. The results show the potential of this approach for characterizing synthetic peptides of relatively high molecular weight.

    Original languageEnglish (US)
    Pages (from-to)727-733
    Number of pages7
    JournalBiological Mass Spectrometry
    Volume23
    Issue number12
    DOIs
    StatePublished - Jan 1 1994

    Fingerprint

    Fast Atom Bombardment Mass Spectrometry
    Electrospray ionization
    Mass spectrometry
    Atoms
    Peptides
    Peptide Fragments
    Electrospray Ionization Mass Spectrometry
    Liquid chromatography
    Liquid Chromatography
    Digestion
    Molecular Weight
    Molecular weight

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Medicine

    Cite this

    Structural characterization of synthetic model peptides of the DNA‐binding cI434 repressor by electrospray ionization and fast atom bombardment mass spectrometry. / Percipalle, Piergiorgio; Saletti, Rosaria; Pongor, Sandor; Foti, Salvatore; Tossi, Alessandro; Fisichella, Salvatore.

    In: Biological Mass Spectrometry, Vol. 23, No. 12, 01.01.1994, p. 727-733.

    Research output: Contribution to journalArticle

    Percipalle, Piergiorgio ; Saletti, Rosaria ; Pongor, Sandor ; Foti, Salvatore ; Tossi, Alessandro ; Fisichella, Salvatore. / Structural characterization of synthetic model peptides of the DNA‐binding cI434 repressor by electrospray ionization and fast atom bombardment mass spectrometry. In: Biological Mass Spectrometry. 1994 ; Vol. 23, No. 12. pp. 727-733.
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    AU - Pongor, Sandor

    AU - Foti, Salvatore

    AU - Tossi, Alessandro

    AU - Fisichella, Salvatore

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