Structural basis for double-stranded RNA processing by Dicer

Ian J. MacRae, Kaihong Zhou, Fei Li, Adrian Repic, Angela N. Brooks, W. Zacheus Cande, Paul D. Adams, Jennifer A. Doudna

Research output: Contribution to journalArticle

Abstract

The specialized ribonuclease Dicer initiates RNA interference by cleaving double-stranded RNA (dsRNA) substrates into small fragments about 25 nucleotides in length. In the crystal structure of an intact Dicer enzyme, the PAZ domain, a module that binds the end of dsRNA, is separated from the two catalytic ribonuclease III (RNase III) domains by a flat, positively charged surface. The 65 angstrom distance between the PAZ and RNase III domains matches the length spanned by 25 base pairs of RNA. Thus, Dicer itself is a molecular ruler that recognizes dsRNA and cleaves a specified distance from the helical end.

Original languageEnglish (US)
Pages (from-to)195-198
Number of pages4
JournalScience
Volume311
Issue number5758
DOIs
StatePublished - Jan 13 2006

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Ribonuclease III
Double-Stranded RNA
Ribonucleases
RNA Interference
Base Pairing
Nucleotides
RNA

ASJC Scopus subject areas

  • General

Cite this

MacRae, I. J., Zhou, K., Li, F., Repic, A., Brooks, A. N., Cande, W. Z., ... Doudna, J. A. (2006). Structural basis for double-stranded RNA processing by Dicer. Science, 311(5758), 195-198. https://doi.org/10.1126/science.1121638

Structural basis for double-stranded RNA processing by Dicer. / MacRae, Ian J.; Zhou, Kaihong; Li, Fei; Repic, Adrian; Brooks, Angela N.; Cande, W. Zacheus; Adams, Paul D.; Doudna, Jennifer A.

In: Science, Vol. 311, No. 5758, 13.01.2006, p. 195-198.

Research output: Contribution to journalArticle

MacRae, IJ, Zhou, K, Li, F, Repic, A, Brooks, AN, Cande, WZ, Adams, PD & Doudna, JA 2006, 'Structural basis for double-stranded RNA processing by Dicer', Science, vol. 311, no. 5758, pp. 195-198. https://doi.org/10.1126/science.1121638
MacRae IJ, Zhou K, Li F, Repic A, Brooks AN, Cande WZ et al. Structural basis for double-stranded RNA processing by Dicer. Science. 2006 Jan 13;311(5758):195-198. https://doi.org/10.1126/science.1121638
MacRae, Ian J. ; Zhou, Kaihong ; Li, Fei ; Repic, Adrian ; Brooks, Angela N. ; Cande, W. Zacheus ; Adams, Paul D. ; Doudna, Jennifer A. / Structural basis for double-stranded RNA processing by Dicer. In: Science. 2006 ; Vol. 311, No. 5758. pp. 195-198.
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