Structural analyses of polyelectrolyte sequence domains within the adhesive elastomeric biomineralization protein Lustrin A

Brandon A. Wustman, Daniel E. Morse, John Evans

Research output: Contribution to journalArticle

Abstract

The lustrin superfamily represents a unique group of biomineralization proteins localized between layered aragonite mineral plates (i.e., nacre layers) in mollusk shell. These proteins exhibit elastomeric and adhesive behavior within the mineralized matrix. One member of the lustrin superfamily, Lustrin A, has been sequenced; the protein is organized into defined, modular sequence domains that are hypothesized to perform separate functions (e.g., force unfolding, mineral interaction, intermolecular binding) within the Lustrin A protein. Using NMR, CD spectrometry, and model peptides, we investigated the solution structure of two Lustrin A polyelectrolyte modular domains that represent putative sites for Lustrin A-nacre component interaction: the 30-AA Arg, Lys, Tyr, Ser-rich (RKSY), and 24-AA Asp-rich (D4) domains. The results indicate that both sequences adopt open unfolded structures, with RKSY exhibiting structural features of an extended conformer, and D4, a more labile, random-coil conformation. These results suggests that the Lustrin A protein possesses open, unfolded regions that could act as putative sites for Lustrin A-mineral or Lustrin A-macromolecular interactions that lead to the observed adhesive properties of the nacre organic matrix.

Original languageEnglish (US)
Pages (from-to)9901-9906
Number of pages6
JournalLangmuir
Volume18
Issue number25
DOIs
StatePublished - Dec 10 2002

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Biomineralization
Polyelectrolytes
adhesives
Adhesives
Nacre
proteins
Proteins
Minerals
minerals
mollusks
aragonite
interactions
matrices
Spectrometry
Peptides
peptides
Conformations
Calcium Carbonate
coils
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

Structural analyses of polyelectrolyte sequence domains within the adhesive elastomeric biomineralization protein Lustrin A. / Wustman, Brandon A.; Morse, Daniel E.; Evans, John.

In: Langmuir, Vol. 18, No. 25, 10.12.2002, p. 9901-9906.

Research output: Contribution to journalArticle

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