Strong stimulation of N2 fixation in oligotrophic Mediterranean Sea: Results from dust addition in large in situ mesocosms

C. Ridame, Cecile Guieu, S. L'Helguen

Research output: Contribution to journalArticle

Abstract

The response of N2 (dinitrogen) fixation to contrasted (wet and dry) Saharan dust deposition was studied in the framework of the DUNE project (a DUst experiment in a low-Nutrient, low-chlorophyll Ecosystem) during which realistic simulations of dust deposition (10 g m−2) into large mesocosms (52 m3) were performed. Three distinct experimental dust additions were conducted in June 2008 (DUNE-1-P: simulation of a wet deposition, DUNE-1-Q: simulation of a dry deposition) and 2010 (DUNE-2-R: simulation of 2 successive wet depositions) in the northwestern oligotrophic Mediterranean Sea. Here we show that wet and dry dust deposition induced a rapid (24 h or 48 h after dust additions), strong (from 2- to 5.3-fold) and long (at least 4-6 days duration) increase in N2 fixation, indicating that both wet and dry Saharan dust deposition was able to relieve efficiently the nutrient limitation(s) of N2 fixation. This means in particular that N2 fixation activity was not inhibited by the significant input of nitrate associated with the simulated wet deposition (∼ 9 mmol NO3 -m -2). The input of new nitrogen associated with N2 fixation was negligible relative to the atmospheric NO3− input associated with the dust. The contribution of N2 fixation to primary production was negligible (≤ 1%) before and after dust addition in all experiments, indicating that N2 fixation was a poor contributor to the nitrogen demand for primary production. Despite the stimulation of N2 fixation by dust addition, the rates remained low, and did not significantly change the contribution of N2 fixation to new production since only a maximum contribution of 10% was observed. The response of N2 fixation by diazotrophs and CO2 fixation by the whole phytoplankton community suggests that these metabolic processes were limited or co-limited by different nutrients. With this novel approach, which allows us to study processes as a function of time while atmospheric particles are sinking, we show that new atmospheric nutrients associated with Saharan dust pulses do significantly stimulate N 2 fixation in the Mediterranean Sea and that N2 fixation is not a key process in the carbon cycle in such oligotrophic environments.

Original languageEnglish (US)
Pages (from-to)7333-7346
Number of pages14
JournalBiogeosciences
Volume10
Issue number11
DOIs
StatePublished - Nov 25 2013

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Mediterranean Sea
dust
fixation
wet deposition
nutrients
primary productivity
in situ
simulation
primary production
nutrient
dry deposition
nitrogen-fixing bacteria
oligotrophic environment
nitrogen
atmospheric particle
nitrogen fixation
nutrient limitation
carbon cycle
phytoplankton
nitrates

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Earth-Surface Processes

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Strong stimulation of N2 fixation in oligotrophic Mediterranean Sea : Results from dust addition in large in situ mesocosms. / Ridame, C.; Guieu, Cecile; L'Helguen, S.

In: Biogeosciences, Vol. 10, No. 11, 25.11.2013, p. 7333-7346.

Research output: Contribution to journalArticle

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