Streptococcus mutans and Streptococcus sobrinus colonization and caries experience in 3- and 5-year-old Thai children

P. Saraithong, K. Pattanaporn, Z. Chen, S. Khongkhunthian, P. Laohapensang, N. Chhun, W. Pattanaporn, H. Y. Gaw, Yihong Li

Research output: Contribution to journalArticle

Abstract

Objectives: The aim of this study was to examine the colonization of Streptococcus mutans and Streptococcus sobrinus in supra-gingival plaque samples and to determine their correlation with the prevalence of early childhood caries (ECC) in Thai children. Materials and methods: A total of 344 Thai children, ages 3 and 5 years, were invited to participate in this study. Caries status of the children was examined. Supra-gingival plaque samples were collected. Quantitative real-time PCR was performed to evaluate DNA levels of S. mutans and S. sobrinus. Results: Eighty-five percent of the children were colonized by S. mutans and 50.9 % of them were colonized by S. sobrinus. The prevalence of ECC was 43.8 % and 56.2 % among 3- and 5-year-old children, respectively, and was significantly associated with the presence of S. mutans and S. sobrinus. The severity of ECC was significantly correlated with increased DNA levels of the two bacteria. Children who were positive for S. mutans and S. sobrinus (Sm+/Sb+) were 8 times or 44 times more likely to experience ECC than children who were Sm−/Sb + or were Sm−/Sb−. Conclusions: The study evidence further suggest that children colonized by both S. mutans and S. sobrinus are at the higher risk for ECC. Clinical relevance: Molecular-based qPCR can be used to detect and quantify S. mutans and S. sobrinus colonization for epidemiological and clinical studies for ECC risk assessment.

Original languageEnglish (US)
Pages (from-to)1955-1964
Number of pages10
JournalClinical Oral Investigations
Volume19
Issue number8
DOIs
StatePublished - Mar 11 2015

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Streptococcus sobrinus
Streptococcus mutans
DNA
Real-Time Polymerase Chain Reaction
Epidemiologic Studies
Bacteria

Keywords

  • Caries risk
  • Early childhood caries
  • Epidemiology
  • Mutans streptococci
  • Preschool children
  • Quantitative real-time PCR

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Streptococcus mutans and Streptococcus sobrinus colonization and caries experience in 3- and 5-year-old Thai children. / Saraithong, P.; Pattanaporn, K.; Chen, Z.; Khongkhunthian, S.; Laohapensang, P.; Chhun, N.; Pattanaporn, W.; Gaw, H. Y.; Li, Yihong.

In: Clinical Oral Investigations, Vol. 19, No. 8, 11.03.2015, p. 1955-1964.

Research output: Contribution to journalArticle

Saraithong, P, Pattanaporn, K, Chen, Z, Khongkhunthian, S, Laohapensang, P, Chhun, N, Pattanaporn, W, Gaw, HY & Li, Y 2015, ' Streptococcus mutans and Streptococcus sobrinus colonization and caries experience in 3- and 5-year-old Thai children', Clinical Oral Investigations, vol. 19, no. 8, pp. 1955-1964. https://doi.org/10.1007/s00784-015-1437-0
Saraithong, P. ; Pattanaporn, K. ; Chen, Z. ; Khongkhunthian, S. ; Laohapensang, P. ; Chhun, N. ; Pattanaporn, W. ; Gaw, H. Y. ; Li, Yihong. / Streptococcus mutans and Streptococcus sobrinus colonization and caries experience in 3- and 5-year-old Thai children. In: Clinical Oral Investigations. 2015 ; Vol. 19, No. 8. pp. 1955-1964.
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abstract = "Objectives: The aim of this study was to examine the colonization of Streptococcus mutans and Streptococcus sobrinus in supra-gingival plaque samples and to determine their correlation with the prevalence of early childhood caries (ECC) in Thai children. Materials and methods: A total of 344 Thai children, ages 3 and 5 years, were invited to participate in this study. Caries status of the children was examined. Supra-gingival plaque samples were collected. Quantitative real-time PCR was performed to evaluate DNA levels of S. mutans and S. sobrinus. Results: Eighty-five percent of the children were colonized by S. mutans and 50.9 {\%} of them were colonized by S. sobrinus. The prevalence of ECC was 43.8 {\%} and 56.2 {\%} among 3- and 5-year-old children, respectively, and was significantly associated with the presence of S. mutans and S. sobrinus. The severity of ECC was significantly correlated with increased DNA levels of the two bacteria. Children who were positive for S. mutans and S. sobrinus (Sm+/Sb+) were 8 times or 44 times more likely to experience ECC than children who were Sm−/Sb + or were Sm−/Sb−. Conclusions: The study evidence further suggest that children colonized by both S. mutans and S. sobrinus are at the higher risk for ECC. Clinical relevance: Molecular-based qPCR can be used to detect and quantify S. mutans and S. sobrinus colonization for epidemiological and clinical studies for ECC risk assessment.",
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AU - Pattanaporn, K.

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AU - Khongkhunthian, S.

AU - Laohapensang, P.

AU - Chhun, N.

AU - Pattanaporn, W.

AU - Gaw, H. Y.

AU - Li, Yihong

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AB - Objectives: The aim of this study was to examine the colonization of Streptococcus mutans and Streptococcus sobrinus in supra-gingival plaque samples and to determine their correlation with the prevalence of early childhood caries (ECC) in Thai children. Materials and methods: A total of 344 Thai children, ages 3 and 5 years, were invited to participate in this study. Caries status of the children was examined. Supra-gingival plaque samples were collected. Quantitative real-time PCR was performed to evaluate DNA levels of S. mutans and S. sobrinus. Results: Eighty-five percent of the children were colonized by S. mutans and 50.9 % of them were colonized by S. sobrinus. The prevalence of ECC was 43.8 % and 56.2 % among 3- and 5-year-old children, respectively, and was significantly associated with the presence of S. mutans and S. sobrinus. The severity of ECC was significantly correlated with increased DNA levels of the two bacteria. Children who were positive for S. mutans and S. sobrinus (Sm+/Sb+) were 8 times or 44 times more likely to experience ECC than children who were Sm−/Sb + or were Sm−/Sb−. Conclusions: The study evidence further suggest that children colonized by both S. mutans and S. sobrinus are at the higher risk for ECC. Clinical relevance: Molecular-based qPCR can be used to detect and quantify S. mutans and S. sobrinus colonization for epidemiological and clinical studies for ECC risk assessment.

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