Strategies for three-dimensional particle tracking with holographic video microscopy

Fook Chiong Cheong, Bhaskar Jyoti Krishnatreya, David G. Grier

    Research output: Contribution to journalArticle

    Abstract

    The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution. In this work, we compare the performance of two complementary analysis techniques, one based on fitting to the exact Lorenz-Mie theory and the other based on phenomenological interpretation of the scattered light field reconstructed with Rayleigh-Sommerfeld back-propagation. Although Lorenz-Mie tracking provides more information and is inherently more precise, Rayleigh-Sommerfeld reconstruction is faster and more general. The two techniques agree quantitatively on colloidal spheres' in-plane positions. Their systematic differences in axial tracking can be explained in terms of the illuminated objects' light scattering properties.

    Original languageEnglish (US)
    Pages (from-to)13563-13573
    Number of pages11
    JournalOptics Express
    Volume18
    Issue number13
    DOIs
    StatePublished - Jun 21 2010

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    three dimensional motion
    microscopy
    Mie scattering
    light scattering
    microscopes

    ASJC Scopus subject areas

    • Atomic and Molecular Physics, and Optics

    Cite this

    Strategies for three-dimensional particle tracking with holographic video microscopy. / Cheong, Fook Chiong; Krishnatreya, Bhaskar Jyoti; Grier, David G.

    In: Optics Express, Vol. 18, No. 13, 21.06.2010, p. 13563-13573.

    Research output: Contribution to journalArticle

    Cheong, Fook Chiong ; Krishnatreya, Bhaskar Jyoti ; Grier, David G. / Strategies for three-dimensional particle tracking with holographic video microscopy. In: Optics Express. 2010 ; Vol. 18, No. 13. pp. 13563-13573.
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