Starfish oocytes form intracellular ice at unusually high temperatures

Murat Köseolu, Ali Erolu, Mehmet Toner, Kirsten Sadler Edepli

    Research output: Contribution to journalArticle

    Abstract

    Starfish oocytes, eggs, and embryos are popular models for studying meiotic maturation, fertilization, and embryonic development. Their large (170- to 200-μm) oocytes are obtainable in copious amounts and are amenable to manipulations that mammalian oocytes are not. The most formidable obstacle to working with marine oocytes is their seasonal availability, yet a successful means of preserving them for use during the nonreproductive season has not been reported. The aim of this study was to investigate the response of starfish oocytes to freezing with rapid and slow cooling rates under a variety of conditions to develop a cryopreservation protocol for these cells. Cryomicroscopic observation revealed that starfish oocytes in isotonic medium undergo intracellular ice formation (IIF) at very high subzero temperatures, such that the mean difference between the temperature of extracellular ice formation (TEIF) and IIF (T11F) was less than 3°C and the average T11F was approximately between -4 and -6°C. Neither partial cellular dehydration nor addition of the cryopreservative dimethyl sulfoxide significantly depressed the T11F. Under some conditions, we observed ice nucleation at multiple locations within the cytoplasm, suggesting that several factors contribute to the unusually high T11F during controlled-rate freezing and thus vitrification may be a more suitable method for cryopreserving these cells.

    Original languageEnglish (US)
    Pages (from-to)248-259
    Number of pages12
    JournalCryobiology
    Volume43
    Issue number3
    DOIs
    StatePublished - Apr 6 2002

    Fingerprint

    Starfish
    Asteroidea
    Ice
    Oocytes
    oocytes
    ice
    Temperature
    Freezing
    temperature
    Vitrification
    freezing
    Dimethyl Sulfoxide
    Dehydration
    ice nucleation
    Nucleation
    Cryopreservation
    vitrification
    Availability
    dimethyl sulfoxide
    Cooling

    Keywords

    • Cryopreservation
    • Freezing
    • Intracellular ice formation
    • Starfish oocytes

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

    Cite this

    Köseolu, M., Erolu, A., Toner, M., & Sadler Edepli, K. (2002). Starfish oocytes form intracellular ice at unusually high temperatures. Cryobiology, 43(3), 248-259. https://doi.org/10.1006/cryo.2001.2348

    Starfish oocytes form intracellular ice at unusually high temperatures. / Köseolu, Murat; Erolu, Ali; Toner, Mehmet; Sadler Edepli, Kirsten.

    In: Cryobiology, Vol. 43, No. 3, 06.04.2002, p. 248-259.

    Research output: Contribution to journalArticle

    Köseolu, M, Erolu, A, Toner, M & Sadler Edepli, K 2002, 'Starfish oocytes form intracellular ice at unusually high temperatures', Cryobiology, vol. 43, no. 3, pp. 248-259. https://doi.org/10.1006/cryo.2001.2348
    Köseolu, Murat ; Erolu, Ali ; Toner, Mehmet ; Sadler Edepli, Kirsten. / Starfish oocytes form intracellular ice at unusually high temperatures. In: Cryobiology. 2002 ; Vol. 43, No. 3. pp. 248-259.
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