Stage-specific transcriptome and proteome analyses of the filarial parasite Onchocerca volvulus and its Wolbachia endosymbiont

Sasisekhar Bennuru, James A. Cotton, Jose M C Ribeiro, Alexandra Grote, Bhavana Harsha, Nancy Holroyd, Amruta Mhashilkar, Douglas M. Molina, Arlo Z. Randall, Adam D. Shandling, Thomas R. Unnasch, Elodie Ghedin, Matthew Berriman, Sara Lustigman, Thomas B. Nutman

Research output: Contribution to journalArticle

Abstract

Onchocerciasis (river blindness) is a neglected tropical disease that has been successfully targeted by mass drug treatment programs in the Americas and small parts of Africa. Achieving the long-term goal of elimination of onchocerciasis, however, requires additional tools, including drugs, vaccines, and biomarkers of infection. Here, we describe the transcriptome and proteome profiles of the major vector and the human host stages (L1, L2, L3, molting L3, L4, adult male, and adult female) of Onchocerca volvulus along with the proteome of each parasitic stage and of its Wolbachia endosymbiont (wOv). In so doing, we have identified stage-specific pathways important to the parasite’s adaptation to its human host during its early development. Further, we generated a protein array that, when screened with well-characterized human samples, identified novel diagnostic biomarkers of O. volvulus infection and new potential vaccine candidates. This immunomic approach not only demonstrates the power of this postgenomic discovery platform but also provides additional tools for onchocerciasis control programs. IMPORTANCE The global onchocerciasis (river blindness) elimination program will have to rely on the development of new tools (drugs, vaccines, biomarkers) to achieve its goals by 2025. As an adjunct to the completed genomic sequencing of O. volvulus, we used a comprehensive proteomic and transcriptomic profiling strategy to gain a comprehensive understanding of both the vector-derived and human host-derived parasite stages. In so doing, we have identified proteins and pathways that enable novel drug targeting studies and the discovery of novel vaccine candidates, as well as useful biomarkers of active infection.

Original languageEnglish (US)
Article numbere02028-16
JournalmBio
Volume7
Issue number6
DOIs
StatePublished - Jan 1 2016

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Wolbachia
Onchocerca volvulus
Onchocerciasis
Gene Expression Profiling
Proteome
Parasites
Ocular Onchocerciasis
Vaccines
Biomarkers
Infection
Pharmaceutical Preparations
Neglected Diseases
Molting
Protein Array Analysis
Drug Delivery Systems
Transcriptome
Proteomics
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Virology

Cite this

Bennuru, S., Cotton, J. A., Ribeiro, J. M. C., Grote, A., Harsha, B., Holroyd, N., ... Nutman, T. B. (2016). Stage-specific transcriptome and proteome analyses of the filarial parasite Onchocerca volvulus and its Wolbachia endosymbiont. mBio, 7(6), [e02028-16]. https://doi.org/10.1128/mBio.02028-16

Stage-specific transcriptome and proteome analyses of the filarial parasite Onchocerca volvulus and its Wolbachia endosymbiont. / Bennuru, Sasisekhar; Cotton, James A.; Ribeiro, Jose M C; Grote, Alexandra; Harsha, Bhavana; Holroyd, Nancy; Mhashilkar, Amruta; Molina, Douglas M.; Randall, Arlo Z.; Shandling, Adam D.; Unnasch, Thomas R.; Ghedin, Elodie; Berriman, Matthew; Lustigman, Sara; Nutman, Thomas B.

In: mBio, Vol. 7, No. 6, e02028-16, 01.01.2016.

Research output: Contribution to journalArticle

Bennuru, S, Cotton, JA, Ribeiro, JMC, Grote, A, Harsha, B, Holroyd, N, Mhashilkar, A, Molina, DM, Randall, AZ, Shandling, AD, Unnasch, TR, Ghedin, E, Berriman, M, Lustigman, S & Nutman, TB 2016, 'Stage-specific transcriptome and proteome analyses of the filarial parasite Onchocerca volvulus and its Wolbachia endosymbiont', mBio, vol. 7, no. 6, e02028-16. https://doi.org/10.1128/mBio.02028-16
Bennuru, Sasisekhar ; Cotton, James A. ; Ribeiro, Jose M C ; Grote, Alexandra ; Harsha, Bhavana ; Holroyd, Nancy ; Mhashilkar, Amruta ; Molina, Douglas M. ; Randall, Arlo Z. ; Shandling, Adam D. ; Unnasch, Thomas R. ; Ghedin, Elodie ; Berriman, Matthew ; Lustigman, Sara ; Nutman, Thomas B. / Stage-specific transcriptome and proteome analyses of the filarial parasite Onchocerca volvulus and its Wolbachia endosymbiont. In: mBio. 2016 ; Vol. 7, No. 6.
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