Stabilizing Leaf and Branch Compost Cutinase (LCC) with Glycosylation: Mechanism and Effect on PET Hydrolysis

Abhijit N. Shirke, Christine White, Jacob A. Englaender, Allison Zwarycz, Glenn Butterfoss, Robert J. Linhardt, Richard A. Gross

Research output: Contribution to journalArticle

Abstract

Cutinases are polyester hydrolases that show a remarkable capability to hydrolyze polyethylene terephthalate (PET) to its monomeric units. This revelation has stimulated research aimed at developing sustainable and green cutinase-catalyzed PET recycling methods. Leaf and branch compost cutinase (LCC) is particularly suited toward these ends given its relatively high PET hydrolysis activity and thermostability. Any practical enzymatic PET recycling application will require that the protein have kinetic stability at or above the PET glass transition temperature (Tg, i.e., 70 °C). This paper elucidates the thermodynamics and kinetics of LCC conformational and colloidal stability. Aggregation emerged as a major contributor that reduces LCC kinetic stability. In its native state, LCC is prone to aggregation owing to electrostatic interactions. Further, with increasing temperature, perturbation of LCC's tertiary structure and corresponding exposure of hydrophobic domains leads to rapid aggregation. Glycosylation was employed in an attempt to impede LCC aggregation. Owing to the presence of three putative N-glycosylation sites, expression of native LCC in Pichia pastoris resulted in the production of glycosylated LCC (LCC-G). LCC-G showed improved stability to native state aggregation while increasing the temperature for thermal induced aggregation by 10 °C. Furthermore, stabilization against thermal aggregation resulted in improved catalytic PET hydrolysis both at its optimum temperature and concentration.

Original languageEnglish (US)
Pages (from-to)1190-1200
Number of pages11
JournalBiochemistry
Volume57
Issue number7
DOIs
StatePublished - Feb 20 2018

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Glycosylation
Polyethylene Terephthalates
Hydrolysis
Soil
Agglomeration
Recycling
Kinetics
Temperature
cutinase
Hot Temperature
Polyesters
Pichia
Transition Temperature
Hydrolases
Coulomb interactions
Static Electricity
Thermodynamics
Glass
Stabilization

ASJC Scopus subject areas

  • Biochemistry

Cite this

Shirke, A. N., White, C., Englaender, J. A., Zwarycz, A., Butterfoss, G., Linhardt, R. J., & Gross, R. A. (2018). Stabilizing Leaf and Branch Compost Cutinase (LCC) with Glycosylation: Mechanism and Effect on PET Hydrolysis. Biochemistry, 57(7), 1190-1200. https://doi.org/10.1021/acs.biochem.7b01189

Stabilizing Leaf and Branch Compost Cutinase (LCC) with Glycosylation : Mechanism and Effect on PET Hydrolysis. / Shirke, Abhijit N.; White, Christine; Englaender, Jacob A.; Zwarycz, Allison; Butterfoss, Glenn; Linhardt, Robert J.; Gross, Richard A.

In: Biochemistry, Vol. 57, No. 7, 20.02.2018, p. 1190-1200.

Research output: Contribution to journalArticle

Shirke, Abhijit N. ; White, Christine ; Englaender, Jacob A. ; Zwarycz, Allison ; Butterfoss, Glenn ; Linhardt, Robert J. ; Gross, Richard A. / Stabilizing Leaf and Branch Compost Cutinase (LCC) with Glycosylation : Mechanism and Effect on PET Hydrolysis. In: Biochemistry. 2018 ; Vol. 57, No. 7. pp. 1190-1200.
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