Spontaneous formation of structurally diverse membrane channel architectures from a single antimicrobial peptide

Yukun Wang, Charles H. Chen, Dan Hu, Martin B. Ulmschneider, Jakob P. Ulmschneider

Research output: Contribution to journalArticle

Abstract

Many antimicrobial peptides (AMPs) selectively target and form pores in microbial membranes. However, the mechanisms of membrane targeting, pore formation and function remain elusive. Here we report an experimentally guided unbiased simulation methodology that yields the mechanism of spontaneous pore assembly for the AMP maculatin at atomic resolution. Rather than a single pore, maculatin forms an ensemble of structurally diverse temporarily functional low-oligomeric pores, which mimic integral membrane protein channels in structure. These pores continuously form and dissociate in the membrane. Membrane permeabilization is dominated by hexa-, hepta-and octamers, which conduct water, ions and small dyes. Pores form by consecutive addition of individual helices to a transmembrane helix or helix bundle, in contrast to current poration models. The diversity of the pore architectures-formed by a single sequence-may be a key feature in preventing bacterial resistance and could explain why sequence-function relationships in AMPs remain elusive.

Original languageEnglish (US)
Article number13535
JournalNature Communications
Volume7
DOIs
StatePublished - Nov 22 2016

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Ion Channels
peptides
membranes
Membranes
porosity
Peptides
helices
Membrane Proteins
Coloring Agents
Ions
Water
bundles
assembly
dyes
methodology
proteins
water
ions

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Spontaneous formation of structurally diverse membrane channel architectures from a single antimicrobial peptide. / Wang, Yukun; Chen, Charles H.; Hu, Dan; Ulmschneider, Martin B.; Ulmschneider, Jakob P.

In: Nature Communications, Vol. 7, 13535, 22.11.2016.

Research output: Contribution to journalArticle

Wang, Yukun ; Chen, Charles H. ; Hu, Dan ; Ulmschneider, Martin B. ; Ulmschneider, Jakob P. / Spontaneous formation of structurally diverse membrane channel architectures from a single antimicrobial peptide. In: Nature Communications. 2016 ; Vol. 7.
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