Sphingolipids stimulate cell growth via MAP kinase activation in osteoblastic cells

L. C. Carpio, E. Stephan, Angela Kamer, R. Dziak

Research output: Contribution to journalArticle

Abstract

Ceramide, ceramide-1-phosphate (C1P) sphingosine (SPH) and sphingosine-1-phosphate (S1P) effects on proliferation and extracellular-signal regulated kinases, ERKs (also known as MAPKs), activation were investigated in human and rat osteoblastic cells. MAPK activation was sphingolipid-specific in cells from both species. In human osteoblastic cells, S1P and C1P markedly stimulated ERK2 phosphorylation with a slight increase in phosphorylation of ERK1. SPH nor ceramide induced phosphorylation of either ERK isoform. In rat osteoblastic cells, SIP, ceramide and SPH stimulated phosphorylation of both isoforms. C1P did not induce phosphorylation of ERK1 but produced a mild increase in phosphorylation of ERK2. In human cells, only S1P significantly (P < 0.05) increased osteoblastic cell proliferation, while in the rat cells all four sphingolipids significantly (P < 0.05) induced proliferation. The calcium channel blocker verapamil blocked (P < 0.05) these effects in both cell types. The MAPK inhibitor, PD98059, inhibited (P < 0.05) the mitogenic effect of SIP in human cells. In rat cells, PD98059 effects were less substantial but significant for S1P and C1P. This study demonstrates that sphingolipids are mitogens for both human and rat osteoblastic cells with the MAPK pathway and calcium mediating in part these effects in a species specific manner.

Original languageEnglish (US)
Pages (from-to)267-273
Number of pages7
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume61
Issue number5
DOIs
StatePublished - Nov 1999

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Sphingolipids
Phosphorylation
Cell growth
Phosphotransferases
Chemical activation
Rats
Sphingosine
Ceramides
Growth
Protein Isoforms
Cells
Extracellular Signal-Regulated MAP Kinases
Cell proliferation
Calcium Channel Blockers
Verapamil
Mitogens
Calcium
sphingosine 1-phosphate
ceramide 1-phosphate
Cell Proliferation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Sphingolipids stimulate cell growth via MAP kinase activation in osteoblastic cells. / Carpio, L. C.; Stephan, E.; Kamer, Angela; Dziak, R.

In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 61, No. 5, 11.1999, p. 267-273.

Research output: Contribution to journalArticle

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