Spectroscopic properties of complexes of acridine orange with glycosaminoglycans. I. Soluble complexes

Mary Cowman, W. B. Rippon, E. W. Abrahamson

Research output: Contribution to journalArticle

Abstract

The interaction of acridine orange with dermatan and chondroitin sulfates results in the formation of complexes containing bound dye molecules ordered into dyssymmetric arrays. Complexes containing an excess of available disaccharide residues compared to dye are completely soluble, and exhibit biphasic circular dichroism bands. Analysis of the dependence of the extrinsic circular dichroism on dye aggregation indicates the presence of extended dye stacks bound to the glycosaminoglycan. Complexes formed in solutions containing an excess of dye are only partially soluble, and exhibit circular dichroism spectra having band shifts and intensity changes relative to the soluble complexes. The latter complexes show a sharp drop in induced circular dichroism with temperature, due to a cooperative change in the structure of the complex. The structural order of the dye glycosaminoglycan complex may differ from the intrinsic structure of the glycosaminoglycan itself in dilute solution.

Original languageEnglish (US)
Pages (from-to)1213-1227
Number of pages15
JournalBiopolymers - Peptide Science Section
Volume15
Issue number6
StatePublished - 1976

Fingerprint

Acridine Orange
Glycosaminoglycans
Coloring Agents
Dyes
Dichroism
Circular Dichroism
Dermatan Sulfate
Chondroitin Sulfates
Disaccharides
Agglomeration
Molecules
Temperature

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Spectroscopic properties of complexes of acridine orange with glycosaminoglycans. I. Soluble complexes. / Cowman, Mary; Rippon, W. B.; Abrahamson, E. W.

In: Biopolymers - Peptide Science Section, Vol. 15, No. 6, 1976, p. 1213-1227.

Research output: Contribution to journalArticle

@article{632c2dff3c3641fe8b431455fb04929c,
title = "Spectroscopic properties of complexes of acridine orange with glycosaminoglycans. I. Soluble complexes",
abstract = "The interaction of acridine orange with dermatan and chondroitin sulfates results in the formation of complexes containing bound dye molecules ordered into dyssymmetric arrays. Complexes containing an excess of available disaccharide residues compared to dye are completely soluble, and exhibit biphasic circular dichroism bands. Analysis of the dependence of the extrinsic circular dichroism on dye aggregation indicates the presence of extended dye stacks bound to the glycosaminoglycan. Complexes formed in solutions containing an excess of dye are only partially soluble, and exhibit circular dichroism spectra having band shifts and intensity changes relative to the soluble complexes. The latter complexes show a sharp drop in induced circular dichroism with temperature, due to a cooperative change in the structure of the complex. The structural order of the dye glycosaminoglycan complex may differ from the intrinsic structure of the glycosaminoglycan itself in dilute solution.",
author = "Mary Cowman and Rippon, {W. B.} and Abrahamson, {E. W.}",
year = "1976",
language = "English (US)",
volume = "15",
pages = "1213--1227",
journal = "Biopolymers",
issn = "0006-3525",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Spectroscopic properties of complexes of acridine orange with glycosaminoglycans. I. Soluble complexes

AU - Cowman, Mary

AU - Rippon, W. B.

AU - Abrahamson, E. W.

PY - 1976

Y1 - 1976

N2 - The interaction of acridine orange with dermatan and chondroitin sulfates results in the formation of complexes containing bound dye molecules ordered into dyssymmetric arrays. Complexes containing an excess of available disaccharide residues compared to dye are completely soluble, and exhibit biphasic circular dichroism bands. Analysis of the dependence of the extrinsic circular dichroism on dye aggregation indicates the presence of extended dye stacks bound to the glycosaminoglycan. Complexes formed in solutions containing an excess of dye are only partially soluble, and exhibit circular dichroism spectra having band shifts and intensity changes relative to the soluble complexes. The latter complexes show a sharp drop in induced circular dichroism with temperature, due to a cooperative change in the structure of the complex. The structural order of the dye glycosaminoglycan complex may differ from the intrinsic structure of the glycosaminoglycan itself in dilute solution.

AB - The interaction of acridine orange with dermatan and chondroitin sulfates results in the formation of complexes containing bound dye molecules ordered into dyssymmetric arrays. Complexes containing an excess of available disaccharide residues compared to dye are completely soluble, and exhibit biphasic circular dichroism bands. Analysis of the dependence of the extrinsic circular dichroism on dye aggregation indicates the presence of extended dye stacks bound to the glycosaminoglycan. Complexes formed in solutions containing an excess of dye are only partially soluble, and exhibit circular dichroism spectra having band shifts and intensity changes relative to the soluble complexes. The latter complexes show a sharp drop in induced circular dichroism with temperature, due to a cooperative change in the structure of the complex. The structural order of the dye glycosaminoglycan complex may differ from the intrinsic structure of the glycosaminoglycan itself in dilute solution.

UR - http://www.scopus.com/inward/record.url?scp=0017082378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017082378&partnerID=8YFLogxK

M3 - Article

C2 - 131589

AN - SCOPUS:0017082378

VL - 15

SP - 1213

EP - 1227

JO - Biopolymers

JF - Biopolymers

SN - 0006-3525

IS - 6

ER -