Spectral Studies of a Chlorophyll Pigment with Fluorescence Maximum at 698 mμ

S. B. Broyde, S. S. Brody

Research output: Contribution to journalArticle

Abstract

A chlorophyll type pigment (F698) fluorescing maximally at 698 mμ at 77°K has been observed in preparations of chlorophyll. This fluorescence is quenched by small amounts of naturally occurring materials, including plastoquinone and the ubiquinones, and by nitrobenzene, probably by formation of a nonfluorescent complex. Fluorescence quenching does not occur in the presence of carotenes, xanthophylls, or reduced plastoquinone and ubiquinone. The fluorescence is sharply temperature dependent, with a steep rise in intensity occurring at 165°K. At 77°K the fluorescence yield is between 0.8 and 1.0. The red absorption maximum of the pigment is at 675 mμ at room temperature and at 688 mμ at 77°K. In vivo, a low temperature emission is also observed at 698 mμ, and this fluorescence is quenched by nitrobenzene. It is proposed that the pigment found in vitro is also the one responsible for emission at 698 mμ in vivo. A reaction of F698 with plastoquinone is suggested as the primary photochemical step in system II of photosynthesis.

Original languageEnglish (US)
Pages (from-to)353-366
Number of pages14
JournalBiophysical journal
Volume6
Issue number3
DOIs
StatePublished - Jan 1 1966

ASJC Scopus subject areas

  • Biophysics

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