Specification of the otic placode depends on Sox9 function in Xenopus

Natasha Saint-Germain, Young Hoon Lee, Yanhui Zhang, Thomas D. Sargent, Jean-Pierre Saint-Jeannet

Research output: Contribution to journalArticle

Abstract

The vertebrate inner ear develops from a thickening of the embryonic ectoderm, adjacent to the hindbrain, known as the otic placode. All components of the inner ear derive from the embryonic otic placode. Sox proteins form a large class of transcriptional regulators implicated in the control of a variety of developmental processes. One member of this family, Sox9, is expressed in the developing inner ear, but little is known about the early function of Sox9 in this tissue. We report the functional analysis of Sox9 during development of Xenopus inner ear. Sox9 otic expression is initiated shortly after gastrulation in the sensory layer of the ectoderm, in a bilateral patch of cells immediately adjacent to the cranial neural crest. In the otic placode, Sox9 colocalizes with Pax8 one of the earliest gene expressed in response to otic placode inducing signals. Depletion of Sox9 protein in whole embryos using morpholino antisense oligonucleotides causes a dramatic loss of the early otic placode markers Pax8 and Tbx2. Later in embryogenesis, Sox9 morpholino-injected embryos lack a morphologically recognizable otic vesicle and fail to express late otic markers (Tbx2, Bmp4, Otx2 and Wnt3a) that normally exhibit regionalized expression pattern throughout the otocyst. Using a hormone inducible inhibitory mutant of Sox9, we demonstrate that Sox9 function is required for otic placode specification but not for its subsequent patterning. We propose that Sox9 is one of the key regulators of inner ear specification in Xenopus.

Original languageEnglish (US)
Pages (from-to)1755-1763
Number of pages9
JournalDevelopment
Volume131
Issue number8
DOIs
StatePublished - Apr 2004

Fingerprint

Xenopus
Ear
Inner Ear
Morpholinos
Ectoderm
Embryonic Structures
Gastrulation
Rhombencephalon
Neural Crest
Antisense Oligonucleotides
Embryonic Development
Vertebrates
Proteins
Hormones

Keywords

  • Dlx3
  • Fgf
  • Inner ear
  • Otic placode
  • Pax8
  • Sox9
  • Tbx2
  • Wnt
  • Xenopus

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Specification of the otic placode depends on Sox9 function in Xenopus. / Saint-Germain, Natasha; Lee, Young Hoon; Zhang, Yanhui; Sargent, Thomas D.; Saint-Jeannet, Jean-Pierre.

In: Development, Vol. 131, No. 8, 04.2004, p. 1755-1763.

Research output: Contribution to journalArticle

Saint-Germain, N, Lee, YH, Zhang, Y, Sargent, TD & Saint-Jeannet, J-P 2004, 'Specification of the otic placode depends on Sox9 function in Xenopus', Development, vol. 131, no. 8, pp. 1755-1763. https://doi.org/10.1242/dev.01066
Saint-Germain, Natasha ; Lee, Young Hoon ; Zhang, Yanhui ; Sargent, Thomas D. ; Saint-Jeannet, Jean-Pierre. / Specification of the otic placode depends on Sox9 function in Xenopus. In: Development. 2004 ; Vol. 131, No. 8. pp. 1755-1763.
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