Specific threonine phosphorylation of a host target by two unrelated type III effectors activates a host innate immune receptor in plants

Eui Hwan Chung, Luis Da Cunha, Ai Jiuan Wu, Zhiyong Gao, Karen Cherkis, Ahmed Afzal, David MacKey, Jeffery L. Dangl

Research output: Contribution to journalArticle

Abstract

The Arabidopsis NB-LRR immune receptor RPM1 recognizes the Pseudomonas syringae type III effectors AvrB or AvrRpm1 to mount an immune response. Although neither effector is itself a kinase, AvrRpm1 and AvrB are known to target Arabidopsis RIN4, a negative regulator of basal plant defense, for phosphorylation. We show that RIN4 phosphorylation activates RPM1. RIN4 142-176 is necessary and, with appropriate localization sequences, sufficient to support effector-triggered RPM1 activation, with the threonine residue at position 166 being critical. Phosphomimic substitutions at T166 cause effector-independent RPM1 activation. RIN4 T166 is phosphorylated in vivo in the presence of AvrB or AvrRpm1. RIN4 mutants that lose interaction with AvrB cannot be coimmunoprecipitated with RPM1. This defines a common interaction platform required for RPM1 activation by phosphorylated RIN4 in response to pathogenic effectors. Conservation of an analogous threonine across all RIN4-like proteins suggests a key function for this residue beyond the regulation of RPM1.

Original languageEnglish (US)
Pages (from-to)125-136
Number of pages12
JournalCell Host and Microbe
Volume9
Issue number2
DOIs
StatePublished - Feb 17 2011

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Threonine
Arabidopsis
Phosphorylation
Pseudomonas syringae
Phosphotransferases
Proteins

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

Cite this

Specific threonine phosphorylation of a host target by two unrelated type III effectors activates a host innate immune receptor in plants. / Chung, Eui Hwan; Da Cunha, Luis; Wu, Ai Jiuan; Gao, Zhiyong; Cherkis, Karen; Afzal, Ahmed; MacKey, David; Dangl, Jeffery L.

In: Cell Host and Microbe, Vol. 9, No. 2, 17.02.2011, p. 125-136.

Research output: Contribution to journalArticle

Chung, Eui Hwan ; Da Cunha, Luis ; Wu, Ai Jiuan ; Gao, Zhiyong ; Cherkis, Karen ; Afzal, Ahmed ; MacKey, David ; Dangl, Jeffery L. / Specific threonine phosphorylation of a host target by two unrelated type III effectors activates a host innate immune receptor in plants. In: Cell Host and Microbe. 2011 ; Vol. 9, No. 2. pp. 125-136.
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AU - Gao, Zhiyong

AU - Cherkis, Karen

AU - Afzal, Ahmed

AU - MacKey, David

AU - Dangl, Jeffery L.

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