Specialized odorant receptors in social insects that detect cuticular hydrocarbon cues and candidate pheromones

Gregory M. Pask, Jesse D. Slone, Jocelyn G. Millar, Prithwiraj Das, Jardel A. Moreira, Xiaofan Zhou, Jan Bello, Shelley L. Berger, Roberto Bonasio, Claude Desplan, Danny Reinberg, Jürgen Liebig, Laurence J. Zwiebel, Anandasankar Ray

Research output: Contribution to journalArticle

Abstract

Eusocial insects use cuticular hydrocarbons as components of pheromones that mediate social behaviours, such as caste and nestmate recognition, and regulation of reproduction. In ants such as Harpegnathos saltator, the queen produces a pheromone which suppresses the development of workers' ovaries and if she is removed, workers can transition to a reproductive state known as gamergate. Here we functionally characterize a subfamily of odorant receptors (Ors) with a nine-exon gene structure that have undergone a massive expansion in ants and other eusocial insects. We deorphanize 22 representative members and find they can detect cuticular hydrocarbons from different ant castes, with one (HsOr263) that responds strongly to gamergate extract and a candidate queen pheromone component. After systematic testing with a diverse panel of hydrocarbons, we find that most Harpegnathos saltator Ors are narrowly tuned, suggesting that several receptors must contribute to detection and discrimination of different cuticular hydrocarbons important in mediating eusocial behaviour.

Original languageEnglish (US)
Article number297
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017

Fingerprint

Odorant Receptors
insects
Pheromones
cues
Hydrocarbons
Cues
Ants
Insects
hydrocarbons
Social Class
ovaries
Social Behavior
genes
Reproduction
discrimination
Ovary
Exons
Genes
expansion
Testing

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Specialized odorant receptors in social insects that detect cuticular hydrocarbon cues and candidate pheromones. / Pask, Gregory M.; Slone, Jesse D.; Millar, Jocelyn G.; Das, Prithwiraj; Moreira, Jardel A.; Zhou, Xiaofan; Bello, Jan; Berger, Shelley L.; Bonasio, Roberto; Desplan, Claude; Reinberg, Danny; Liebig, Jürgen; Zwiebel, Laurence J.; Ray, Anandasankar.

In: Nature Communications, Vol. 8, No. 1, 297, 01.12.2017.

Research output: Contribution to journalArticle

Pask, GM, Slone, JD, Millar, JG, Das, P, Moreira, JA, Zhou, X, Bello, J, Berger, SL, Bonasio, R, Desplan, C, Reinberg, D, Liebig, J, Zwiebel, LJ & Ray, A 2017, 'Specialized odorant receptors in social insects that detect cuticular hydrocarbon cues and candidate pheromones', Nature Communications, vol. 8, no. 1, 297. https://doi.org/10.1038/s41467-017-00099-1
Pask, Gregory M. ; Slone, Jesse D. ; Millar, Jocelyn G. ; Das, Prithwiraj ; Moreira, Jardel A. ; Zhou, Xiaofan ; Bello, Jan ; Berger, Shelley L. ; Bonasio, Roberto ; Desplan, Claude ; Reinberg, Danny ; Liebig, Jürgen ; Zwiebel, Laurence J. ; Ray, Anandasankar. / Specialized odorant receptors in social insects that detect cuticular hydrocarbon cues and candidate pheromones. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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