Sox10 regulates the development of neural crest-derived melanocytes in Xenopus

Yoichiro Aoki, Natasha Saint-Germain, Michael Gyda, Emily Magner-Fink, Young Hoon Lee, Christine Credidio, Jean-Pierre Saint-Jeannet

Research output: Contribution to journalArticle

Abstract

The transcription factors of the Sox family play important roles in diverse developmental processes. A number of genetic studies have established that Sox10 is a major regulator of neural crest formation. Here, we report the cloning and functional analysis of the Xenopus Sox10 gene. Sox10 mRNA accumulates during gastrulation at the lateral edges of the neural plate, in the neural crest-forming region. In this tissue, Sox10 expression is regulated by Wnt signaling and colocalizes with two major regulators of neural crest formation, Slug and Sox9. While initially expressed in neural crest cells from all axial levels, at the tailbud stage, Sox10 is downregulated in the cranial neural crest and persists mostly in neural crest cells from the trunk region. Overexpression of Sox10 causes a dramatic expansion of the Slug expression domain. We show that the C-terminal portion of Sox10 is sufficient to mediate this activity. Later during embryogenesis, Sox10-injected embryos show a massive increase in pigment cells (Trp-2-expressing cells). The responsiveness of the embryo to Sox10 overexpression by expansion of the Slug expression domain and ectopic production of Trp-2-positive cells and differentiated melanocytes is lost during gastrulation, as revealed by a hormone-inducible Sox10 construct. These results suggest that Sox10 is involved in the specification of neural crest progenitors fated to form the pigment cell lineage.

Original languageEnglish (US)
Pages (from-to)19-33
Number of pages15
JournalDevelopmental Biology
Volume259
Issue number1
DOIs
StatePublished - Jul 1 2003

Fingerprint

Neural Crest
Melanocytes
Xenopus
Gastropoda
Gastrulation
Embryonic Structures
Neural Plate
Cell Lineage
Embryonic Development
Organism Cloning
Transcription Factors
Down-Regulation
Hormones
Messenger RNA
Genes

Keywords

  • Melanocytes
  • Neural crest
  • Slug
  • Sox10
  • Sox9
  • Trp-2
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Sox10 regulates the development of neural crest-derived melanocytes in Xenopus. / Aoki, Yoichiro; Saint-Germain, Natasha; Gyda, Michael; Magner-Fink, Emily; Lee, Young Hoon; Credidio, Christine; Saint-Jeannet, Jean-Pierre.

In: Developmental Biology, Vol. 259, No. 1, 01.07.2003, p. 19-33.

Research output: Contribution to journalArticle

Aoki, Y, Saint-Germain, N, Gyda, M, Magner-Fink, E, Lee, YH, Credidio, C & Saint-Jeannet, J-P 2003, 'Sox10 regulates the development of neural crest-derived melanocytes in Xenopus', Developmental Biology, vol. 259, no. 1, pp. 19-33. https://doi.org/10.1016/S0012-1606(03)00161-1
Aoki Y, Saint-Germain N, Gyda M, Magner-Fink E, Lee YH, Credidio C et al. Sox10 regulates the development of neural crest-derived melanocytes in Xenopus. Developmental Biology. 2003 Jul 1;259(1):19-33. https://doi.org/10.1016/S0012-1606(03)00161-1
Aoki, Yoichiro ; Saint-Germain, Natasha ; Gyda, Michael ; Magner-Fink, Emily ; Lee, Young Hoon ; Credidio, Christine ; Saint-Jeannet, Jean-Pierre. / Sox10 regulates the development of neural crest-derived melanocytes in Xenopus. In: Developmental Biology. 2003 ; Vol. 259, No. 1. pp. 19-33.
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