Sonic hedgehog is required for progenitor cell maintenance in telencephalic stem cell niches

Robert Machold, Shigemi Hayashi, Michael Rutlin, Mandar D. Muzumdar, Susana Nery, Joshua G. Corbin, Amel Gritli-Linde, Tammy Dellovade, Jeffery A. Porter, Lee L. Rubin, Henryk Dudek, Andrew P. McMahon, Gordon Fishell

Research output: Contribution to journalArticle

Abstract

To directly test the requirement for hedgehog signaling in the telencephalon from early neurogenesis, we examined conditional null alleles of both the Sonic hedgehog and Smoothened genes. While the removal of Shh signaling in these animals resulted in only minor patterning abnormalities, the number of neural progenitors in both the postnatal subventricular zone and hippocampus was dramatically reduced. In the subventricular zone, this was partially attributable to a marked increase in programmed cell death. Consistent with Hedgehog signaling being required for the maintenance of stem cell niches in the adult brain, progenitors from the subventricular zone of floxed Smo animals formed significantly fewer neurospheres. The loss of hedgehog signaling also resulted in abnormalities in the dentate gyrus and olfactory bulb. Furthermore, stimulation of the hedgehog pathway in the mature brain resulted in elevated proliferation in telencephalic progenitors. These results suggest that hedgehog signaling is required to maintain progenitor cells in the postnatal telencephalon.

Original languageEnglish (US)
Pages (from-to)937-950
Number of pages14
JournalNeuron
Volume39
Issue number6
DOIs
StatePublished - Sep 11 2003

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Stem Cell Niche
Telencephalon
Hedgehogs
Stem Cells
Maintenance
Lateral Ventricles
Olfactory Bulb
Neurogenesis
Dentate Gyrus
Brain
Hippocampus
Cell Death
Alleles
Genes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Machold, R., Hayashi, S., Rutlin, M., Muzumdar, M. D., Nery, S., Corbin, J. G., ... Fishell, G. (2003). Sonic hedgehog is required for progenitor cell maintenance in telencephalic stem cell niches. Neuron, 39(6), 937-950. https://doi.org/10.1016/S0896-6273(03)00561-0

Sonic hedgehog is required for progenitor cell maintenance in telencephalic stem cell niches. / Machold, Robert; Hayashi, Shigemi; Rutlin, Michael; Muzumdar, Mandar D.; Nery, Susana; Corbin, Joshua G.; Gritli-Linde, Amel; Dellovade, Tammy; Porter, Jeffery A.; Rubin, Lee L.; Dudek, Henryk; McMahon, Andrew P.; Fishell, Gordon.

In: Neuron, Vol. 39, No. 6, 11.09.2003, p. 937-950.

Research output: Contribution to journalArticle

Machold, R, Hayashi, S, Rutlin, M, Muzumdar, MD, Nery, S, Corbin, JG, Gritli-Linde, A, Dellovade, T, Porter, JA, Rubin, LL, Dudek, H, McMahon, AP & Fishell, G 2003, 'Sonic hedgehog is required for progenitor cell maintenance in telencephalic stem cell niches', Neuron, vol. 39, no. 6, pp. 937-950. https://doi.org/10.1016/S0896-6273(03)00561-0
Machold R, Hayashi S, Rutlin M, Muzumdar MD, Nery S, Corbin JG et al. Sonic hedgehog is required for progenitor cell maintenance in telencephalic stem cell niches. Neuron. 2003 Sep 11;39(6):937-950. https://doi.org/10.1016/S0896-6273(03)00561-0
Machold, Robert ; Hayashi, Shigemi ; Rutlin, Michael ; Muzumdar, Mandar D. ; Nery, Susana ; Corbin, Joshua G. ; Gritli-Linde, Amel ; Dellovade, Tammy ; Porter, Jeffery A. ; Rubin, Lee L. ; Dudek, Henryk ; McMahon, Andrew P. ; Fishell, Gordon. / Sonic hedgehog is required for progenitor cell maintenance in telencephalic stem cell niches. In: Neuron. 2003 ; Vol. 39, No. 6. pp. 937-950.
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