Sonic hedgehog expressing and responding cells generate neuronal diversity in the medial amygdala

Rosalind S.E. Carney, Jean Marie Mangin, Lindsay Hayes, Kevin Mansfield, Vitor H. Sousa, Gordon Fishell, Robert P. Machold, Sohyun Ahn, Vittorio Gallo, Joshua G. Corbin

Research output: Contribution to journalArticle

Abstract

Background: The mammalian amygdala is composed of two primary functional subdivisions, classified according to whether the major output projection of each nucleus is excitatory or inhibitory. The posterior dorsal and ventral subdivisions of the medial amygdala, which primarily contain inhibitory output neurons, modulate specific aspects of innate socio-sexual and aggressive behaviors. However, the development of the neuronal diversity of this complex and important structure remains to be fully elucidated.Results: Using a combination of genetic fate-mapping and loss-of-function analyses, we examined the contribution and function of Sonic hedgehog (Shh)-expressing and Shh-responsive (Nkx2-1+ and Gli1+) neurons in the medial amygdala. Specifically, we found that Shh- and Nkx2-1-lineage cells contribute differentially to the dorsal and ventral subdivisions of the postnatal medial amygdala. These Shh- and Nkx2-1-lineage neurons express overlapping and non-overlapping inhibitory neuronal markers, such as Calbindin, FoxP2, nNOS and Somatostatin, revealing diverse fate contributions in discrete medial amygdala nuclear subdivisions. Electrophysiological analysis of the Shh-derived neurons additionally reveals an important functional diversity within this lineage in the medial amygdala. Moreover, inducible Gli1CreER(T2) temporal fate mapping shows that early-generated progenitors that respond to Shh signaling also contribute to medial amygdala neuronal diversity. Lastly, analysis of Nkx2-1 mutant mice demonstrates a genetic requirement for Nkx2-1 in inhibitory neuronal specification in the medial amygdala distinct from the requirement for Nkx2-1 in cerebral cortical development.Conclusions: Taken together, these data reveal a differential contribution of Shh-expressing and Shh-responding cells to medial amygdala neuronal diversity as well as the function of Nkx2-1 in the development of this important limbic system structure.

Original languageEnglish (US)
Article number14
JournalNeural Development
Volume5
Issue number1
DOIs
StatePublished - May 27 2010

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Hedgehogs
Amygdala
Neurons
Calbindins
Limbic System
Somatostatin
Sexual Behavior

ASJC Scopus subject areas

  • Developmental Neuroscience

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Carney, R. S. E., Mangin, J. M., Hayes, L., Mansfield, K., Sousa, V. H., Fishell, G., ... Corbin, J. G. (2010). Sonic hedgehog expressing and responding cells generate neuronal diversity in the medial amygdala. Neural Development, 5(1), [14]. https://doi.org/10.1186/1749-8104-5-14

Sonic hedgehog expressing and responding cells generate neuronal diversity in the medial amygdala. / Carney, Rosalind S.E.; Mangin, Jean Marie; Hayes, Lindsay; Mansfield, Kevin; Sousa, Vitor H.; Fishell, Gordon; Machold, Robert P.; Ahn, Sohyun; Gallo, Vittorio; Corbin, Joshua G.

In: Neural Development, Vol. 5, No. 1, 14, 27.05.2010.

Research output: Contribution to journalArticle

Carney, RSE, Mangin, JM, Hayes, L, Mansfield, K, Sousa, VH, Fishell, G, Machold, RP, Ahn, S, Gallo, V & Corbin, JG 2010, 'Sonic hedgehog expressing and responding cells generate neuronal diversity in the medial amygdala', Neural Development, vol. 5, no. 1, 14. https://doi.org/10.1186/1749-8104-5-14
Carney, Rosalind S.E. ; Mangin, Jean Marie ; Hayes, Lindsay ; Mansfield, Kevin ; Sousa, Vitor H. ; Fishell, Gordon ; Machold, Robert P. ; Ahn, Sohyun ; Gallo, Vittorio ; Corbin, Joshua G. / Sonic hedgehog expressing and responding cells generate neuronal diversity in the medial amygdala. In: Neural Development. 2010 ; Vol. 5, No. 1.
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AU - Mangin, Jean Marie

AU - Hayes, Lindsay

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AU - Sousa, Vitor H.

AU - Fishell, Gordon

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