Somatodendritic microRNAs identified by laser capture and multiplex RT-PCR

Min Jeong Kye, Tsunglin Liu, Sasha F. Levy, Lan Xu Nan, Benjamin B. Groves, Richard Bonneau, Kaiqin Lao, Kenneth S. Kosik

Research output: Contribution to journalArticle

Abstract

The catalog of RNAs present in dendrites represents the complete repertoire of local translation that contributes to synaptic plasticity. Most views hold that a pool of dendritic mRNAs is selectively transported to a dendritic destination. This view requires that some mRNAs in the dendrite are locally enriched relative to the cell body; however, quantitative comparisons that would support this assumption do not currently exist. These issues related to somatodendritic distribution of mRNAs also apply to the microRNAs, ∼21 nucleotide noncoding transcripts that bind to target mRNAs and either inhibit their translation or destabilize them. We combined laser capture with multiplex real-time RT (reverse transcription) PCR to quantify microRNAs in the neuritic and somatic compartments separately. The samples were standardized by RT-PCR measurements of a set of mRNAs, including known dendritic mRNAs, in these two compartments. Most neuronal miRNAs were detected in dendrites. With a few notable exceptions, most miRNAs were distributed through the somatodendritic compartment across a nearly constant gradient. Thus for lower-abundance miRNAs, the total neuronal concentration of the miRNA can remain readily detectable in the cell body but vanish from the dendrite. A very small number of miRNAs deviate from the distribution gradient across the miRNA population as relatively enriched or depleted in the dendrite. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1224-1234
Number of pages11
JournalRNA
Volume13
Issue number8
DOIs
StatePublished - Aug 2007

Fingerprint

MicroRNAs
Reverse Transcription
Lasers
Dendrites
Polymerase Chain Reaction
Messenger RNA
Neuronal Plasticity
Nucleotides
RNA
Population

Keywords

  • Dendritic translation
  • mRNA
  • Plasticity
  • RNA localization
  • Synapses

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Kye, M. J., Liu, T., Levy, S. F., Nan, L. X., Groves, B. B., Bonneau, R., ... Kosik, K. S. (2007). Somatodendritic microRNAs identified by laser capture and multiplex RT-PCR. RNA, 13(8), 1224-1234. https://doi.org/10.1261/rna.480407

Somatodendritic microRNAs identified by laser capture and multiplex RT-PCR. / Kye, Min Jeong; Liu, Tsunglin; Levy, Sasha F.; Nan, Lan Xu; Groves, Benjamin B.; Bonneau, Richard; Lao, Kaiqin; Kosik, Kenneth S.

In: RNA, Vol. 13, No. 8, 08.2007, p. 1224-1234.

Research output: Contribution to journalArticle

Kye, MJ, Liu, T, Levy, SF, Nan, LX, Groves, BB, Bonneau, R, Lao, K & Kosik, KS 2007, 'Somatodendritic microRNAs identified by laser capture and multiplex RT-PCR', RNA, vol. 13, no. 8, pp. 1224-1234. https://doi.org/10.1261/rna.480407
Kye, Min Jeong ; Liu, Tsunglin ; Levy, Sasha F. ; Nan, Lan Xu ; Groves, Benjamin B. ; Bonneau, Richard ; Lao, Kaiqin ; Kosik, Kenneth S. / Somatodendritic microRNAs identified by laser capture and multiplex RT-PCR. In: RNA. 2007 ; Vol. 13, No. 8. pp. 1224-1234.
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