Skeletal Muscle Growth Hormone Receptor Signaling Regulates Basal, but Not Fasting-Induced, Lipid Oxidation

Archana Vijayakumar, YingJie Wu, Nicholas J. Buffin, Xiaosong Li, Hui Sun, Ronald E. Gordon, Shoshana Yakar, Derek LeRoith

Research output: Contribution to journalArticle

Abstract

Background: Growth hormone (GH) stimulates whole-body lipid oxidation, but its regulation of muscle lipid oxidation is not clearly defined. Mice with a skeletal muscle-specific knockout of the GH receptor (mGHRKO model) are protected from high fat diet (HFD)-induced insulin resistance and display increased whole-body carbohydrate utilization. In this study we used the mGRHKO mice to investigate the role of muscle GHR signaling on lipid oxidation under regular chow (RC)- and HFD- fed conditions, and in response to fasting. Methodology/Principal Findings: Expression of lipid oxidation genes was analyzed by real-time PCR in the muscles of RC- and HFD- fed mice, and after 24 h fasting in the HFD-fed mice. Expression of lipid oxidation genes was lower in the muscles of the mGHRKO mice relative to the controls, irrespective of diet. However, in response to 24 h fasting, the HFD-fed mGHRKO mice displayed up-regulation of lipid oxidation genes similar to the fasted controls. When subjected to treadmill running challenge, the HFD-fed mGHRKO mice demonstrated increased whole-body lipid utilization. Additionally, under fasted conditions, the adipose tissue of the mGHRKO mice displayed increased lipolysis as compared to both the fed mGHRKO as well as the fasted control mice. Conclusions/Significance: Our data show that muscle GHR signaling regulates basal lipid oxidation, but not the induction of lipid oxidation in response to fasting. We further demonstrate that muscle GHR signaling is involved in muscle-adipose tissue cross-talk; however the mechanisms mediating this remain to be elucidated.

Original languageEnglish (US)
Article numbere44777
JournalPLoS One
Volume7
Issue number9
DOIs
StatePublished - Sep 14 2012

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Somatotropin Receptors
fasting
Muscle
skeletal muscle
Fasting
Skeletal Muscle
Nutrition
lipid peroxidation
High Fat Diet
high fat diet
Lipids
Oxidation
mice
Fats
Muscles
muscles
Genes
body fat
adipose tissue
Adipose Tissue

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Vijayakumar, A., Wu, Y., Buffin, N. J., Li, X., Sun, H., Gordon, R. E., ... LeRoith, D. (2012). Skeletal Muscle Growth Hormone Receptor Signaling Regulates Basal, but Not Fasting-Induced, Lipid Oxidation. PLoS One, 7(9), [e44777]. https://doi.org/10.1371/journal.pone.0044777

Skeletal Muscle Growth Hormone Receptor Signaling Regulates Basal, but Not Fasting-Induced, Lipid Oxidation. / Vijayakumar, Archana; Wu, YingJie; Buffin, Nicholas J.; Li, Xiaosong; Sun, Hui; Gordon, Ronald E.; Yakar, Shoshana; LeRoith, Derek.

In: PLoS One, Vol. 7, No. 9, e44777, 14.09.2012.

Research output: Contribution to journalArticle

Vijayakumar, Archana ; Wu, YingJie ; Buffin, Nicholas J. ; Li, Xiaosong ; Sun, Hui ; Gordon, Ronald E. ; Yakar, Shoshana ; LeRoith, Derek. / Skeletal Muscle Growth Hormone Receptor Signaling Regulates Basal, but Not Fasting-Induced, Lipid Oxidation. In: PLoS One. 2012 ; Vol. 7, No. 9.
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