Six-helix and eight-helix DNA nanotubes assembled from half-tubes

Akinori Kuzuya, Risheng Wang, Ruojie Sha, Nadrian Seeman

Research output: Contribution to journalArticle

Abstract

DMA nanotubes are cylinder-like structures formed from DNA double-helical molecules whose helix axes are fused at least twice by crossovers. It is potentially useful to use such tubes as sheaths around rodlike species that arise in biological systems and in nanotechnology. It seems easiest to obtain such sheathing by joining two or more components around an object rather than attempting to thread the object through a cavity in the tube. We report two examples of tubes containing a specific number of helices that are assembled from half-tube components. These tubes are a six-helix bundle and an eight-helix bundle, constructed respectively from two bent triple-crossover (BTX) molecules and from two four-helix arched motifs. Both species contain single strands in one molecule that are missing in its mate. The six-helix bundle is formed from two different BTX molecules, whereas the eight-helix species is a closed cyclic dimer of the same molecule. We demonstrate the formation of these species by gel electrophoresis, and we examine their arrangement into long one-dimensional arrays by means of atomic force microscopy.

Original languageEnglish (US)
Pages (from-to)1757-1763
Number of pages7
JournalNano Letters
Volume7
Issue number6
DOIs
StatePublished - Jun 2007

Fingerprint

helices
Nanotubes
nanotubes
DNA
deoxyribonucleic acid
tubes
Molecules
bundles
molecules
crossovers
Biological systems
Dynamic mechanical analysis
Electrophoresis
Nanotechnology
Joining
Dimers
Atomic force microscopy
threads
Gels
electrophoresis

ASJC Scopus subject areas

  • Materials Science(all)
  • Electronic, Optical and Magnetic Materials
  • Chemistry (miscellaneous)

Cite this

Six-helix and eight-helix DNA nanotubes assembled from half-tubes. / Kuzuya, Akinori; Wang, Risheng; Sha, Ruojie; Seeman, Nadrian.

In: Nano Letters, Vol. 7, No. 6, 06.2007, p. 1757-1763.

Research output: Contribution to journalArticle

Kuzuya, Akinori ; Wang, Risheng ; Sha, Ruojie ; Seeman, Nadrian. / Six-helix and eight-helix DNA nanotubes assembled from half-tubes. In: Nano Letters. 2007 ; Vol. 7, No. 6. pp. 1757-1763.
@article{ccb557938e2943858252e000589f056d,
title = "Six-helix and eight-helix DNA nanotubes assembled from half-tubes",
abstract = "DMA nanotubes are cylinder-like structures formed from DNA double-helical molecules whose helix axes are fused at least twice by crossovers. It is potentially useful to use such tubes as sheaths around rodlike species that arise in biological systems and in nanotechnology. It seems easiest to obtain such sheathing by joining two or more components around an object rather than attempting to thread the object through a cavity in the tube. We report two examples of tubes containing a specific number of helices that are assembled from half-tube components. These tubes are a six-helix bundle and an eight-helix bundle, constructed respectively from two bent triple-crossover (BTX) molecules and from two four-helix arched motifs. Both species contain single strands in one molecule that are missing in its mate. The six-helix bundle is formed from two different BTX molecules, whereas the eight-helix species is a closed cyclic dimer of the same molecule. We demonstrate the formation of these species by gel electrophoresis, and we examine their arrangement into long one-dimensional arrays by means of atomic force microscopy.",
author = "Akinori Kuzuya and Risheng Wang and Ruojie Sha and Nadrian Seeman",
year = "2007",
month = "6",
doi = "10.1021/nl070828k",
language = "English (US)",
volume = "7",
pages = "1757--1763",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Six-helix and eight-helix DNA nanotubes assembled from half-tubes

AU - Kuzuya, Akinori

AU - Wang, Risheng

AU - Sha, Ruojie

AU - Seeman, Nadrian

PY - 2007/6

Y1 - 2007/6

N2 - DMA nanotubes are cylinder-like structures formed from DNA double-helical molecules whose helix axes are fused at least twice by crossovers. It is potentially useful to use such tubes as sheaths around rodlike species that arise in biological systems and in nanotechnology. It seems easiest to obtain such sheathing by joining two or more components around an object rather than attempting to thread the object through a cavity in the tube. We report two examples of tubes containing a specific number of helices that are assembled from half-tube components. These tubes are a six-helix bundle and an eight-helix bundle, constructed respectively from two bent triple-crossover (BTX) molecules and from two four-helix arched motifs. Both species contain single strands in one molecule that are missing in its mate. The six-helix bundle is formed from two different BTX molecules, whereas the eight-helix species is a closed cyclic dimer of the same molecule. We demonstrate the formation of these species by gel electrophoresis, and we examine their arrangement into long one-dimensional arrays by means of atomic force microscopy.

AB - DMA nanotubes are cylinder-like structures formed from DNA double-helical molecules whose helix axes are fused at least twice by crossovers. It is potentially useful to use such tubes as sheaths around rodlike species that arise in biological systems and in nanotechnology. It seems easiest to obtain such sheathing by joining two or more components around an object rather than attempting to thread the object through a cavity in the tube. We report two examples of tubes containing a specific number of helices that are assembled from half-tube components. These tubes are a six-helix bundle and an eight-helix bundle, constructed respectively from two bent triple-crossover (BTX) molecules and from two four-helix arched motifs. Both species contain single strands in one molecule that are missing in its mate. The six-helix bundle is formed from two different BTX molecules, whereas the eight-helix species is a closed cyclic dimer of the same molecule. We demonstrate the formation of these species by gel electrophoresis, and we examine their arrangement into long one-dimensional arrays by means of atomic force microscopy.

UR - http://www.scopus.com/inward/record.url?scp=34547379212&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547379212&partnerID=8YFLogxK

U2 - 10.1021/nl070828k

DO - 10.1021/nl070828k

M3 - Article

C2 - 17500580

AN - SCOPUS:34547379212

VL - 7

SP - 1757

EP - 1763

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 6

ER -