Site-specific transgenesis by Cre-mediated recombination in Drosophila

Adam Oberstein, Adam Pare, Leah Kaplan, Stephen Small

Research output: Contribution to journalArticle

Abstract

Transposons such as P elements are routinely used to stably transfer exogenous DNA (transgenes) into the Drosophila genome. Transgene insertion events, however, are essentially random and are subject to 'position effects' from nearby endogenous regulatory elements. Here we describe a microinjection-based system that uses Cre-mediated recombination to insert transgenes into precise genomic 'landing sites'. The system is simple and efficient, and will permit precise comparisons between multiple transgenic constructs.

Original languageEnglish (US)
Pages (from-to)583-585
Number of pages3
JournalNature Methods
Volume2
Issue number8
DOIs
StatePublished - Aug 2005

Fingerprint

Gene Transfer Techniques
Landing
Transgenes
Genetic Recombination
Drosophila
Genes
DNA
Microinjections
Genome

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

Site-specific transgenesis by Cre-mediated recombination in Drosophila. / Oberstein, Adam; Pare, Adam; Kaplan, Leah; Small, Stephen.

In: Nature Methods, Vol. 2, No. 8, 08.2005, p. 583-585.

Research output: Contribution to journalArticle

Oberstein, Adam ; Pare, Adam ; Kaplan, Leah ; Small, Stephen. / Site-specific transgenesis by Cre-mediated recombination in Drosophila. In: Nature Methods. 2005 ; Vol. 2, No. 8. pp. 583-585.
@article{18764d8dc3b84972bb92cfe0c16699b6,
title = "Site-specific transgenesis by Cre-mediated recombination in Drosophila",
abstract = "Transposons such as P elements are routinely used to stably transfer exogenous DNA (transgenes) into the Drosophila genome. Transgene insertion events, however, are essentially random and are subject to 'position effects' from nearby endogenous regulatory elements. Here we describe a microinjection-based system that uses Cre-mediated recombination to insert transgenes into precise genomic 'landing sites'. The system is simple and efficient, and will permit precise comparisons between multiple transgenic constructs.",
author = "Adam Oberstein and Adam Pare and Leah Kaplan and Stephen Small",
year = "2005",
month = "8",
doi = "10.1038/nmeth775",
language = "English (US)",
volume = "2",
pages = "583--585",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Public Library of Science",
number = "8",

}

TY - JOUR

T1 - Site-specific transgenesis by Cre-mediated recombination in Drosophila

AU - Oberstein, Adam

AU - Pare, Adam

AU - Kaplan, Leah

AU - Small, Stephen

PY - 2005/8

Y1 - 2005/8

N2 - Transposons such as P elements are routinely used to stably transfer exogenous DNA (transgenes) into the Drosophila genome. Transgene insertion events, however, are essentially random and are subject to 'position effects' from nearby endogenous regulatory elements. Here we describe a microinjection-based system that uses Cre-mediated recombination to insert transgenes into precise genomic 'landing sites'. The system is simple and efficient, and will permit precise comparisons between multiple transgenic constructs.

AB - Transposons such as P elements are routinely used to stably transfer exogenous DNA (transgenes) into the Drosophila genome. Transgene insertion events, however, are essentially random and are subject to 'position effects' from nearby endogenous regulatory elements. Here we describe a microinjection-based system that uses Cre-mediated recombination to insert transgenes into precise genomic 'landing sites'. The system is simple and efficient, and will permit precise comparisons between multiple transgenic constructs.

UR - http://www.scopus.com/inward/record.url?scp=23144438746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23144438746&partnerID=8YFLogxK

U2 - 10.1038/nmeth775

DO - 10.1038/nmeth775

M3 - Article

VL - 2

SP - 583

EP - 585

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 8

ER -