Single-molecule detection limits in levitated microdroplets

William B. Whitten, J. Michael Ramsey, Stephen Arnold, Burt V. Bronk

Research output: Contribution to journalArticle

Abstract

Laser-excited fluorescence from electrodynamically levitated microdropiets is used to detect small numbers of rhodamine6G molecules. The small sample volume, typically a few picoliters reduces the background due to solvent and impurity Raman and fluorescence emission. With 514.5-nm excitation from an argon ion laser, as few as 12 molecules have been detected in glycerol-water droplets. Our present detection limit, due to variations in the impurity concentration in the blanks, corresponds to a signal-to-noise ratio of 3 for a single molecule of rhodamine-6G contained in a 1-pL volume (droplet diameter of ≃ 12 μm).

Original languageEnglish (US)
Pages (from-to)1027-1031
Number of pages5
JournalAnalytical Chemistry
Volume63
Issue number10
StatePublished - May 15 1991

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Molecules
Fluorescence
Impurities
Lasers
Argon
Glycerol
Signal to noise ratio
Ions
Water
rhodamine 6G

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Whitten, W. B., Ramsey, J. M., Arnold, S., & Bronk, B. V. (1991). Single-molecule detection limits in levitated microdroplets. Analytical Chemistry, 63(10), 1027-1031.

Single-molecule detection limits in levitated microdroplets. / Whitten, William B.; Ramsey, J. Michael; Arnold, Stephen; Bronk, Burt V.

In: Analytical Chemistry, Vol. 63, No. 10, 15.05.1991, p. 1027-1031.

Research output: Contribution to journalArticle

Whitten, WB, Ramsey, JM, Arnold, S & Bronk, BV 1991, 'Single-molecule detection limits in levitated microdroplets', Analytical Chemistry, vol. 63, no. 10, pp. 1027-1031.
Whitten WB, Ramsey JM, Arnold S, Bronk BV. Single-molecule detection limits in levitated microdroplets. Analytical Chemistry. 1991 May 15;63(10):1027-1031.
Whitten, William B. ; Ramsey, J. Michael ; Arnold, Stephen ; Bronk, Burt V. / Single-molecule detection limits in levitated microdroplets. In: Analytical Chemistry. 1991 ; Vol. 63, No. 10. pp. 1027-1031.
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