Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors

Alexandra Chloé Villani, Rahul Satija, Gary Reynolds, Siranush Sarkizova, Karthik Shekhar, James Fletcher, Morgane Griesbeck, Andrew Butler, Shiwei Zheng, Suzan Lazo, Laura Jardine, David Dixon, Emily Stephenson, Emil Nilsson, Ida Grundberg, David McDonald, Andrew Filby, Weibo Li, Philip L. De Jager, Orit Rozenblatt-Rosen & 4 others Andrew A. Lane, Muzlifah Haniffa, Aviv Regev, Nir Hacohen

Research output: Contribution to journalArticle

Abstract

Dendritic cells (DCs) and monocytes play a central role in pathogen sensing, phagocytosis, and antigen presentation and consist of multiple specialized subtypes. However, their identities and interrelationships are not fully understood. Using unbiased single-cell RNA sequencing (RNA-seq) of ~2400 cells, we identified six human DCs and four monocyte subtypes in human blood. Our study reveals a new DC subset that shares properties with plasmacytoid DCs (pDCs) but potently activates T cells, thus redefining pDCs; a new subdivision within the CD1C+ subset of DCs; the relationship between blastic plasmacytoid DC neoplasia cells and healthy DCs; and circulating progenitor of conventional DCs (cDCs). Our revised taxonomy will enable more accurate functional and developmental analyses as well as immune monitoring in health and disease.

Original languageEnglish (US)
Article numbereaah4573
JournalScience
Volume356
Issue number6335
DOIs
StatePublished - Apr 21 2017

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RNA Sequence Analysis
Dendritic Cells
Monocytes
Blood Cells
Immunologic Monitoring
Antigen Presentation
Phagocytosis
T-Lymphocytes
Health

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Villani, A. C., Satija, R., Reynolds, G., Sarkizova, S., Shekhar, K., Fletcher, J., ... Hacohen, N. (2017). Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. Science, 356(6335), [eaah4573]. https://doi.org/10.1126/science.aah4573

Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. / Villani, Alexandra Chloé; Satija, Rahul; Reynolds, Gary; Sarkizova, Siranush; Shekhar, Karthik; Fletcher, James; Griesbeck, Morgane; Butler, Andrew; Zheng, Shiwei; Lazo, Suzan; Jardine, Laura; Dixon, David; Stephenson, Emily; Nilsson, Emil; Grundberg, Ida; McDonald, David; Filby, Andrew; Li, Weibo; De Jager, Philip L.; Rozenblatt-Rosen, Orit; Lane, Andrew A.; Haniffa, Muzlifah; Regev, Aviv; Hacohen, Nir.

In: Science, Vol. 356, No. 6335, eaah4573, 21.04.2017.

Research output: Contribution to journalArticle

Villani, AC, Satija, R, Reynolds, G, Sarkizova, S, Shekhar, K, Fletcher, J, Griesbeck, M, Butler, A, Zheng, S, Lazo, S, Jardine, L, Dixon, D, Stephenson, E, Nilsson, E, Grundberg, I, McDonald, D, Filby, A, Li, W, De Jager, PL, Rozenblatt-Rosen, O, Lane, AA, Haniffa, M, Regev, A & Hacohen, N 2017, 'Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors', Science, vol. 356, no. 6335, eaah4573. https://doi.org/10.1126/science.aah4573
Villani AC, Satija R, Reynolds G, Sarkizova S, Shekhar K, Fletcher J et al. Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. Science. 2017 Apr 21;356(6335). eaah4573. https://doi.org/10.1126/science.aah4573
Villani, Alexandra Chloé ; Satija, Rahul ; Reynolds, Gary ; Sarkizova, Siranush ; Shekhar, Karthik ; Fletcher, James ; Griesbeck, Morgane ; Butler, Andrew ; Zheng, Shiwei ; Lazo, Suzan ; Jardine, Laura ; Dixon, David ; Stephenson, Emily ; Nilsson, Emil ; Grundberg, Ida ; McDonald, David ; Filby, Andrew ; Li, Weibo ; De Jager, Philip L. ; Rozenblatt-Rosen, Orit ; Lane, Andrew A. ; Haniffa, Muzlifah ; Regev, Aviv ; Hacohen, Nir. / Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. In: Science. 2017 ; Vol. 356, No. 6335.
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AU - Villani, Alexandra Chloé

AU - Satija, Rahul

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AU - Shekhar, Karthik

AU - Fletcher, James

AU - Griesbeck, Morgane

AU - Butler, Andrew

AU - Zheng, Shiwei

AU - Lazo, Suzan

AU - Jardine, Laura

AU - Dixon, David

AU - Stephenson, Emily

AU - Nilsson, Emil

AU - Grundberg, Ida

AU - McDonald, David

AU - Filby, Andrew

AU - Li, Weibo

AU - De Jager, Philip L.

AU - Rozenblatt-Rosen, Orit

AU - Lane, Andrew A.

AU - Haniffa, Muzlifah

AU - Regev, Aviv

AU - Hacohen, Nir

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