Simultaneous epitope and transcriptome measurement in single cells

Marlon Stoeckius, Christoph Hafemeister, William Stephenson, Brian Houck-Loomis, Pratip K. Chattopadhyay, Harold Swerdlow, Rahul Satija, Peter Smibert

Research output: Contribution to journalArticle

Abstract

High-throughput single-cell RNA sequencing has transformed our understanding of complex cell populations, but it does not provide phenotypic information such as cell-surface protein levels. Here, we describe cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), a method in which oligonucleotide-labeled antibodies are used to integrate cellular protein and transcriptome measurements into an efficient, single-cell readout. CITE-seq is compatible with existing single-cell sequencing approaches and scales readily with throughput increases.

Original languageEnglish (US)
Pages (from-to)865-868
Number of pages4
JournalNature Methods
Volume14
Issue number9
DOIs
StatePublished - Aug 31 2017

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Transcriptome
Epitopes
Throughput
Oligonucleotides
Membrane Proteins
Cells
RNA
RNA Sequence Analysis
Antibodies
Proteins
Population

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Stoeckius, M., Hafemeister, C., Stephenson, W., Houck-Loomis, B., Chattopadhyay, P. K., Swerdlow, H., ... Smibert, P. (2017). Simultaneous epitope and transcriptome measurement in single cells. Nature Methods, 14(9), 865-868. https://doi.org/10.1038/nmeth.4380

Simultaneous epitope and transcriptome measurement in single cells. / Stoeckius, Marlon; Hafemeister, Christoph; Stephenson, William; Houck-Loomis, Brian; Chattopadhyay, Pratip K.; Swerdlow, Harold; Satija, Rahul; Smibert, Peter.

In: Nature Methods, Vol. 14, No. 9, 31.08.2017, p. 865-868.

Research output: Contribution to journalArticle

Stoeckius, M, Hafemeister, C, Stephenson, W, Houck-Loomis, B, Chattopadhyay, PK, Swerdlow, H, Satija, R & Smibert, P 2017, 'Simultaneous epitope and transcriptome measurement in single cells', Nature Methods, vol. 14, no. 9, pp. 865-868. https://doi.org/10.1038/nmeth.4380
Stoeckius M, Hafemeister C, Stephenson W, Houck-Loomis B, Chattopadhyay PK, Swerdlow H et al. Simultaneous epitope and transcriptome measurement in single cells. Nature Methods. 2017 Aug 31;14(9):865-868. https://doi.org/10.1038/nmeth.4380
Stoeckius, Marlon ; Hafemeister, Christoph ; Stephenson, William ; Houck-Loomis, Brian ; Chattopadhyay, Pratip K. ; Swerdlow, Harold ; Satija, Rahul ; Smibert, Peter. / Simultaneous epitope and transcriptome measurement in single cells. In: Nature Methods. 2017 ; Vol. 14, No. 9. pp. 865-868.
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