Silk fibroin hydrogels coupled with the n16N-β-chitin complex: An in vitro organic matrix for controlling calcium carbonate mineralization

Ellen C. Keene, John Evans, Lara A. Estroff

Research output: Contribution to journalArticle

Abstract

Previous results have shown that the nacre specific peptide, n16N, from the Japanese pearl oyster Pinctada fucata has a binding affinity for β-chitin. As a result, the n16N-chitin assembly is able to selectivity nucleate aragonite. Here, we have added silk fibroin hydrogels to the in vitro assay to more fully represent the in vivo matrix. Crystallization, with a silk fibroin hydrogel and n16N on β-chitin, results in metastable vaterite and amorphous calcium carbonate, which form as flat deposits with hemispherical centers. Acidic peptide controls (p-Asp/p-Glu) were also tested in the silk-chitin assay and result in flat calcite that grows into the β-chitin substrate. Fluorescence imaging of that matrix, made with labeled n16N, shows that n16N binds to β-chitin in the presence of silk gel. These results demonstrate that the addition of a silk hydrogel to the n16N-β-chitin assembly changes the microenvironment for mineralization. This work contributes to our understanding of the roles of individual nacre matrix components (and their assemblies) in controlling crystal growth.

Original languageEnglish (US)
Pages (from-to)5169-5175
Number of pages7
JournalCrystal Growth and Design
Volume10
Issue number12
DOIs
StatePublished - Dec 1 2010

Fingerprint

Fibroins
chitin
Chitin
silk
Hydrogels
Silk
Calcium Carbonate
calcium carbonates
Calcium carbonate
matrices
Nacre
Hydrogel
Crystallization
Peptides
peptides
Assays
assembly
aragonite
Calcite
calcite

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Silk fibroin hydrogels coupled with the n16N-β-chitin complex : An in vitro organic matrix for controlling calcium carbonate mineralization. / Keene, Ellen C.; Evans, John; Estroff, Lara A.

In: Crystal Growth and Design, Vol. 10, No. 12, 01.12.2010, p. 5169-5175.

Research output: Contribution to journalArticle

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