Signal enhancement in protein NMR using the spin-noise tuning optimum

Martin Nausner, Michael Goger, Eli Bendet-Taicher, Judith Schlagnitweit, Alexej Jerschow, Norbert Müller

Research output: Contribution to journalArticle

Abstract

We have assessed the potential of an alternative probe tuning strategy based on the spin-noise response for application in common high-resolution multi-dimensional biomolecular NMR experiments with water signal suppression on aqueous and salty samples. The method requires the adjustment of the optimal tuning condition, which may be offset by several 100 kHz from the conventional tuning settings using the noise response of the water protons as an indicator. Although the radio frequency-pulse durations are typically longer under such conditions, signal-to-noise gains of up to 22% were achieved. At salt concentrations up to 100 mM a substantial sensitivity gain was observed.

Original languageEnglish (US)
Pages (from-to)157-167
Number of pages11
JournalJournal of Biomolecular NMR
Volume48
Issue number3
DOIs
StatePublished - Nov 1 2010

    Fingerprint

Keywords

  • Absorbed circuit noise
  • Probe tuning
  • SNTO
  • Sensitivity
  • Signal-to-noise gain
  • Spin-noise

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

Cite this

Nausner, M., Goger, M., Bendet-Taicher, E., Schlagnitweit, J., Jerschow, A., & Müller, N. (2010). Signal enhancement in protein NMR using the spin-noise tuning optimum. Journal of Biomolecular NMR, 48(3), 157-167. https://doi.org/10.1007/s10858-010-9446-4