SH3 interactome conserves general function over specific form

Xiaofeng Xin, David Gfeller, Jackie Cheng, Raffi Tonikian, Lin Sun, Ailan Guo, Lianet Lopez, Alevtina Pavlenco, Adenrele Akintobi, Yingnan Zhang, Jean François Rual, Bridget Currell, Somasekar Seshagiri, Tong Hao, Xinping Yang, Yun A. Shen, Kourosh Salehi-Ashtiani, Jingjing Li, Aaron T. Cheng, Dryden BouamalayAdrien Lugari, David E. Hill, Mark L. Grimes, David G. Drubin, Barth D. Grant, Marc Vidal, Charles Boone, Sachdev S. Sidhu, Gary D. Bader

Research output: Contribution to journalArticle

Abstract

Src homology 3 (SH3) domains bind peptides to mediate protein-protein interactions that assemble and regulate dynamic biological processes. We surveyed the repertoire of SH3 binding specificity using peptide phage display in a metazoan, the worm Caenorhabditis elegans, and discovered that it structurally mirrors that of the budding yeast Saccharomyces cerevisiae. We then mapped the worm SH3 interactome using stringent yeast two-hybrid and compared it with the equivalent map for yeast. We found that the worm SH3 interactome resembles the analogous yeast network because it is significantly enriched for proteins with roles in endocytosis. Nevertheless, orthologous SH3 domain-mediated interactions are highly rewired. Our results suggest a model of network evolution where general function of the SH3 domain network is conserved over its specific form.

Original languageEnglish (US)
Article number652
JournalMolecular Systems Biology
Volume9
DOIs
StatePublished - Jan 1 2013

Fingerprint

src Homology Domains
Conserve
Yeast
Homology
Yeasts
yeasts
Worm
Biological Phenomena
Peptides
Proteins
Saccharomycetales
Caenorhabditis elegans
peptides
Endocytosis
Bacteriophages
Saccharomyces cerevisiae
endocytosis
protein-protein interactions
bacteriophages
Network Evolution

Keywords

  • network evolution
  • phage display
  • protein interaction conservation
  • SH3 domains
  • yeast two-hybrid

ASJC Scopus subject areas

  • Information Systems
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)
  • Computational Theory and Mathematics
  • Applied Mathematics

Cite this

Xin, X., Gfeller, D., Cheng, J., Tonikian, R., Sun, L., Guo, A., ... Bader, G. D. (2013). SH3 interactome conserves general function over specific form. Molecular Systems Biology, 9, [652]. https://doi.org/10.1038/msb.2013.9

SH3 interactome conserves general function over specific form. / Xin, Xiaofeng; Gfeller, David; Cheng, Jackie; Tonikian, Raffi; Sun, Lin; Guo, Ailan; Lopez, Lianet; Pavlenco, Alevtina; Akintobi, Adenrele; Zhang, Yingnan; Rual, Jean François; Currell, Bridget; Seshagiri, Somasekar; Hao, Tong; Yang, Xinping; Shen, Yun A.; Salehi-Ashtiani, Kourosh; Li, Jingjing; Cheng, Aaron T.; Bouamalay, Dryden; Lugari, Adrien; Hill, David E.; Grimes, Mark L.; Drubin, David G.; Grant, Barth D.; Vidal, Marc; Boone, Charles; Sidhu, Sachdev S.; Bader, Gary D.

In: Molecular Systems Biology, Vol. 9, 652, 01.01.2013.

Research output: Contribution to journalArticle

Xin, X, Gfeller, D, Cheng, J, Tonikian, R, Sun, L, Guo, A, Lopez, L, Pavlenco, A, Akintobi, A, Zhang, Y, Rual, JF, Currell, B, Seshagiri, S, Hao, T, Yang, X, Shen, YA, Salehi-Ashtiani, K, Li, J, Cheng, AT, Bouamalay, D, Lugari, A, Hill, DE, Grimes, ML, Drubin, DG, Grant, BD, Vidal, M, Boone, C, Sidhu, SS & Bader, GD 2013, 'SH3 interactome conserves general function over specific form', Molecular Systems Biology, vol. 9, 652. https://doi.org/10.1038/msb.2013.9
Xin X, Gfeller D, Cheng J, Tonikian R, Sun L, Guo A et al. SH3 interactome conserves general function over specific form. Molecular Systems Biology. 2013 Jan 1;9. 652. https://doi.org/10.1038/msb.2013.9
Xin, Xiaofeng ; Gfeller, David ; Cheng, Jackie ; Tonikian, Raffi ; Sun, Lin ; Guo, Ailan ; Lopez, Lianet ; Pavlenco, Alevtina ; Akintobi, Adenrele ; Zhang, Yingnan ; Rual, Jean François ; Currell, Bridget ; Seshagiri, Somasekar ; Hao, Tong ; Yang, Xinping ; Shen, Yun A. ; Salehi-Ashtiani, Kourosh ; Li, Jingjing ; Cheng, Aaron T. ; Bouamalay, Dryden ; Lugari, Adrien ; Hill, David E. ; Grimes, Mark L. ; Drubin, David G. ; Grant, Barth D. ; Vidal, Marc ; Boone, Charles ; Sidhu, Sachdev S. ; Bader, Gary D. / SH3 interactome conserves general function over specific form. In: Molecular Systems Biology. 2013 ; Vol. 9.
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