Sexual-stage-specific RNA expression of a new Plasmodium falciparum gene detected by in situ hybridisation

David A. Baker, Joanne Thompson, Olalekan O. Daramola, Jane M R Carlton, Geoffrey A T Targett

Research output: Contribution to journalArticle

Abstract

A gene, Pf77, transcribed in the sexual stages of Plasmodium falciparum was isolated from a genomic expression library with a polyclonal antibody raised to gametocyte proteins. The entire coding region was obtained from a series of overlapping genomic and cDNA clones (from gametocyte RNA). A single open reading frame is present with no introns and no tandem repeat sequences. A Pf77 probe hybridised to a single transcript present in RNA prepared from purified gametocytes and could not be detected in RNA prepared from asexual blood stages. In situ hybridisation studies confirmed that the expression of Pf77 mRNA is sexual-stage-specific and in addition, showed that Pf77 mRNA is present only in female gametocytes during the vertebrate stages of the parasite's development.

Original languageEnglish (US)
Pages (from-to)193-201
Number of pages9
JournalMolecular and Biochemical Parasitology
Volume72
Issue number1-2
DOIs
StatePublished - 1995

Fingerprint

Plasmodium falciparum
In Situ Hybridization
RNA
Genes
Messenger RNA
Tandem Repeat Sequences
Genomic Library
Introns
Open Reading Frames
Vertebrates
Parasites
Complementary DNA
Clone Cells
Antibodies
Proteins

Keywords

  • Gametocyte
  • In situ hybridization
  • Malaria
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Sexual-stage-specific RNA expression of a new Plasmodium falciparum gene detected by in situ hybridisation. / Baker, David A.; Thompson, Joanne; Daramola, Olalekan O.; Carlton, Jane M R; Targett, Geoffrey A T.

In: Molecular and Biochemical Parasitology, Vol. 72, No. 1-2, 1995, p. 193-201.

Research output: Contribution to journalArticle

Baker, David A. ; Thompson, Joanne ; Daramola, Olalekan O. ; Carlton, Jane M R ; Targett, Geoffrey A T. / Sexual-stage-specific RNA expression of a new Plasmodium falciparum gene detected by in situ hybridisation. In: Molecular and Biochemical Parasitology. 1995 ; Vol. 72, No. 1-2. pp. 193-201.
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