Serotonin and its 5-HT1 receptor in human mastocytosis

Markus Ritter, Husameldin El-Nour, Mari Anne Hedblad, Joseph H. Butterfield, Olof Beck, Niclaus Stephanson, Mikael Holst, Ricardo Giscombe, Efrain Azmitia, Klas Nordlind

Research output: Contribution to journalArticle

Abstract

Context: Human mastocytosis is a rare disease, in which the serotonergic system may be involved. Objective: The objective of the present study was to examine the possible presence of serotonin (5-HT) and its 5-HT1A receptor (R) in the skin of patients with mastocytosis. In addition, the effect of the 5-HT1AR was tested on human mastocytosis cells, cultured in vitro. Materials and methods: The expression of 5-HT and 5-HT1AR in patients with urticaria pigmentosa and mastocytoma was studied using immunohistochemistry. The effects of 8-OH-DPAT, an agonist of 5-HT1AR, on the proliferation (cell number), viability, apoptosis, spontaneous release of histamine, as well as a possible 5-HT metabolism, in the human HMC-1 mast cell line, were investigated. Results: Both 5-HT and 5-HT1AR were expressed in the mast cells in biopsies of mastocytoma and urticaria pigmentosa, as well as in HMC-1 cells. However, no metabolism of 5-HT by the cell line could be detected by the methodology used. The 5-HT1AR agonist had no significant effect on the viability and number of HMC-1 cells, and was without effect on the apoptosis. At concentrations of 10-6 mol/L and 10-810-10 mol/L (i.e. also at physiological concentrations), the agonist inhibited histamine release by these cells by as much as 30%. Conclusion: These findings indicate that 5-HT and its 5-HT1AR are expressed in human mastocytosis and that an agonist of the 5-HT1AR might be of value in the treatment of these patients.

Original languageEnglish (US)
Pages (from-to)679-685
Number of pages7
JournalImmunopharmacology and Immunotoxicology
Volume34
Issue number4
DOIs
StatePublished - Aug 2012

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Serotonin 5-HT1 Receptors
Mastocytosis
Serotonin
Urticaria Pigmentosa
Mastocytoma
Histamine Release
Metabolism
Mast Cells
Histamine
Cells
Apoptosis
8-Hydroxy-2-(di-n-propylamino)tetralin
Cell Line
Receptor, Serotonin, 5-HT1A
Biopsy
Cell proliferation
Rare Diseases
Cultured Cells
Cell Survival
Skin

Keywords

  • Human
  • Mast cell
  • Mastocytosis
  • Receptor
  • Serotonin

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology
  • Toxicology

Cite this

Ritter, M., El-Nour, H., Hedblad, M. A., Butterfield, J. H., Beck, O., Stephanson, N., ... Nordlind, K. (2012). Serotonin and its 5-HT1 receptor in human mastocytosis. Immunopharmacology and Immunotoxicology, 34(4), 679-685. https://doi.org/10.3109/08923973.2011.651222

Serotonin and its 5-HT1 receptor in human mastocytosis. / Ritter, Markus; El-Nour, Husameldin; Hedblad, Mari Anne; Butterfield, Joseph H.; Beck, Olof; Stephanson, Niclaus; Holst, Mikael; Giscombe, Ricardo; Azmitia, Efrain; Nordlind, Klas.

In: Immunopharmacology and Immunotoxicology, Vol. 34, No. 4, 08.2012, p. 679-685.

Research output: Contribution to journalArticle

Ritter, M, El-Nour, H, Hedblad, MA, Butterfield, JH, Beck, O, Stephanson, N, Holst, M, Giscombe, R, Azmitia, E & Nordlind, K 2012, 'Serotonin and its 5-HT1 receptor in human mastocytosis', Immunopharmacology and Immunotoxicology, vol. 34, no. 4, pp. 679-685. https://doi.org/10.3109/08923973.2011.651222
Ritter M, El-Nour H, Hedblad MA, Butterfield JH, Beck O, Stephanson N et al. Serotonin and its 5-HT1 receptor in human mastocytosis. Immunopharmacology and Immunotoxicology. 2012 Aug;34(4):679-685. https://doi.org/10.3109/08923973.2011.651222
Ritter, Markus ; El-Nour, Husameldin ; Hedblad, Mari Anne ; Butterfield, Joseph H. ; Beck, Olof ; Stephanson, Niclaus ; Holst, Mikael ; Giscombe, Ricardo ; Azmitia, Efrain ; Nordlind, Klas. / Serotonin and its 5-HT1 receptor in human mastocytosis. In: Immunopharmacology and Immunotoxicology. 2012 ; Vol. 34, No. 4. pp. 679-685.
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AU - Stephanson, Niclaus

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