Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100 beta antisense gene

S. Ueda, E. T. Kokotos Leonardi, 3rd Bell J., E. C. Azmitia

Research output: Contribution to journalArticle

Abstract

S-100 beta, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100 beta in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100 beta antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100 beta from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.
Original languageUndefined
Pages (from-to)365-8
JournalMolecular Brain Research
Volume29
Issue number2
StatePublished - 1995

Keywords

  • Animals Calcium-Binding Proteins/genetics Cell Transplantation *DNA, Antisense Female Gene Expression Regulation, Neoplastic/*physiology Glioma/*genetics Neoplasm Transplantation Nerve Fibers/physiology Nerve Growth Factors/*genetics *Nerve Regeneration Rats Rats, Sprague-Dawley S100 Calcium Binding Protein beta Subunit S100 Proteins/genetics Serotonin/*physiology

Cite this

Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100 beta antisense gene. / Ueda, S.; Kokotos Leonardi, E. T.; Bell J., 3rd; Azmitia, E. C.

In: Molecular Brain Research, Vol. 29, No. 2, 1995, p. 365-8.

Research output: Contribution to journalArticle

Ueda, S. ; Kokotos Leonardi, E. T. ; Bell J., 3rd ; Azmitia, E. C. / Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100 beta antisense gene. In: Molecular Brain Research. 1995 ; Vol. 29, No. 2. pp. 365-8.
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abstract = "S-100 beta, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100 beta in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100 beta antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100 beta from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.",
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AB - S-100 beta, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100 beta in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100 beta antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100 beta from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.

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